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C30 HPLC Column

1. Polymeric C30 alkyl chains
2. Very lipophilic
3. Exceptional selectivity pattern for geometric isomers

Availability: In Stock

Description

Hawach C30 HPLC Columns are reversed-phase chromatography columns packed with high-performance C30 bonded silica stationary phase. Designed for the separation of highly hydrophobic compounds, C30 columns provide excellent selectivity for carotenoids, vitamins, lipids, and other nonpolar analytes that require enhanced shape selectivity.

Available in 3μm, 5μm and 10μm particle sizes with 120Å pore size silica, Hawach C30 HPLC Columns offer reliable chromatographic performance, high efficiency, excellent reproducibility, and compatibility with conventional HPLC and UHPLC systems.

C30 HPLC Column Features

  • C30 bonded silica stationary phase: Provides unique steric selectivity for long-chain hydrophobic molecules and geometric isomers.
  • Excellent carotenoid separation capability: Ideal for lutein, zeaxanthin, beta-carotene, and other carotenoid compounds.
  • High-performance silica support: 120Å pore size silica particles ensure stable mass transfer and efficient separation.
  • Wide particle size selection: Available in 3μm, 5μm, and 10μm configurations for analytical and preparative requirements.
  • Reliable batch-to-batch consistency: Controlled manufacturing process ensures reproducible retention and peak performance.
  • Multiple dimensions available: Compatible with different HPLC platforms and analytical methods.

C30 HPLC Column Technical Specifications

Parameter

Specification

Stationary Phase

C30 Bonded Silica

Mode

Reversed Phase HPLC

Particle Size

3μm / 5μm / 10μm

Pore Size

120Å

Carbon Loading

High C30 Surface Modification

pH Range

2.0 – 8.0

Temperature Limit

≤60°C

Endcapping

Yes

Column Hardware

Stainless Steel

Application

Carotenoids, Vitamins, Lipids, Hydrophobic Compounds

 

Applications

Application Area

Typical Compounds

Carotenoid Analysis

β-Carotene, Lycopene, Lutein, Zeaxanthin

Vitamin Analysis

Vitamin A, Vitamin D, Fat-soluble vitamins

Food & Nutritional Testing

Natural pigments, antioxidants, functional ingredients

Pharmaceutical Analysis

Hydrophobic active pharmaceutical ingredients

Lipid Analysis

Long-chain lipids and nonpolar compounds

Why Choose C30 HPLC Columns?

Unlike conventional C18 reversed-phase columns, C30 stationary phases provide stronger steric selectivity due to their longer alkyl chain structure. This unique surface chemistry improves the separation of structurally similar hydrophobic molecules, especially compounds with different geometric configurations.

C30 HPLC Columns are widely recognized as an effective solution for challenging separations involving carotenoids, lipid-soluble vitamins, and other highly nonpolar substances where traditional reversed-phase columns may provide insufficient selectivity.

Selection Guide

Particle Size

Recommended Use

3μm C30 Column

High efficiency analytical HPLC, faster separation, improved resolution

5μm C30 Column

Routine analytical HPLC methods with excellent reliability

10μm C30 Column

Preparative and semi-preparative chromatography applications

C30 HPLC Column vs C18 HPLC Column

Both C30 and C18 columns are widely used reversed-phase HPLC columns based on alkyl bonded silica. However, the longer C30 alkyl chain provides stronger hydrophobic interaction and enhanced steric selectivity, making C30 columns particularly suitable for complex hydrophobic compounds and structural isomer separations.

Feature

C30 HPLC Column

C18 HPLC Column

Stationary Phase

C30 bonded silica

C18 bonded silica

Carbon Chain Length

C30 long alkyl chain

C18 alkyl chain

Hydrophobicity

Very high

High

Steric Selectivity

Excellent

Moderate

Isomer Separation

Superior performance

Limited for some compounds

Main Applications

Carotenoids, vitamins, lipids, nonpolar compounds

General pharmaceutical, environmental and biochemical analysis

C30 HPLC Column Product Specifications

C30 HPLC Column, 3μm, 120Å

Cat#

Description

Package

13021317

C30, 3μm, 120Å, 2.1×30mm

1 pc/pk

13021318

C30, 3μm, 120Å, 2.1×33mm

1 pc/pk

13021319

C30, 3μm, 120Å, 2.1×50mm

1 pc/pk

13021320

C30, 3μm, 120Å, 2.1×75mm

1 pc/pk

13021321

C30, 3μm, 120Å, 2.1×100mm

1 pc/pk

13021322

C30, 3μm, 120Å, 2.1×125mm

1 pc/pk

13021323

C30, 3μm, 120Å, 2.1×150mm

1 pc/pk

13021324

C30, 3μm, 120Å, 2.1×200mm

1 pc/pk

13021325

C30, 3μm, 120Å, 2.1×250mm

1 pc/pk

Additional dimensions available: 3.0mm, 4.0mm, 4.6mm internal diameter; 30mm–250mm length options

C30 HPLC Column, 5μm, 120Å

Cat#

Description

Package

13021353

C30, 5μm, 120Å, 2.1×30mm

1 pc/pk

13021354

C30, 5μm, 120Å, 2.1×33mm

1 pc/pk

13021355

C30, 5μm, 120Å, 2.1×50mm

1 pc/pk

13021356

C30, 5μm, 120Å, 2.1×75mm

1 pc/pk

13021357

C30, 5μm, 120Å, 2.1×100mm

1 pc/pk

Available dimensions: 2.1mm, 3.0mm, 4.0mm, 4.6mm I.D.; 30mm–300mm length options

C30 HPLC Column, 10μm, 120Å

Cat#

Description

Package

13021392

C30, 10μm, 120Å, 4.6×250mm

1 pc/pk

Download PDF Datasheet

Recommended C30 HPLC Column Dimensions

Column Dimension

Typical Application

2.1 × 30–150mm

UHPLC / LC-MS compatible high efficiency analysis

3.0 × 50–150mm

Analytical HPLC with reduced solvent consumption

4.6 × 150–250mm

Standard analytical HPLC methods

4.6 × 250–300mm

High resolution separation requiring longer column length

Operating Recommendations

  • Use HPLC-grade solvents and filtered mobile phases to maintain column performance.
  • Filter samples before injection to reduce blockage caused by particulate contamination.
  • Avoid sudden pressure changes during column installation and operation.
  • Store the column according to manufacturer recommendations after use.
  • Use appropriate guard columns for applications involving complex sample matrices.

Frequently Asked Questions

What is a C30 HPLC Column?

A C30 HPLC Column is a reversed-phase chromatography column packed with silica particles chemically bonded with C30 alkyl chains. The extended carbon chain provides enhanced hydrophobic retention and steric selectivity for difficult separations.

What are C30 HPLC Columns used for?

C30 columns are commonly used for carotenoid analysis, vitamin analysis, lipid separation, food analysis, nutritional testing and pharmaceutical applications involving hydrophobic compounds.

What is the difference between C30 and C18 HPLC Columns?

Compared with C18 columns, C30 columns provide stronger shape selectivity and improved separation of structural isomers, especially for carotenoids and other highly nonpolar molecules.

Which particle size should I choose for a C30 column?

3μm C30 columns are suitable for high efficiency analysis and fast separations, while 5μm C30 columns are commonly selected for routine HPLC methods. 10μm C30 columns are designed for applications requiring larger loading capacity.

Hawach C30 HPLC Columns Quality Assurance

Each Hawach C30 HPLC Column is manufactured under controlled production conditions with strict quality testing to ensure consistent retention behavior, column efficiency and chromatographic reproducibility.

With flexible particle sizes, dimensions and reliable batch performance, Hawach C30 HPLC Columns provide a dependable solution for analytical laboratories, research institutions and industrial testing applications.

ISO9001 CE Certificates
The quality has a guarantee

The quality has a guarantee

Fast & safe shipping worldwide

Fast & safe shipping worldwide

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