PPT 96-well Protein Precipitation Filter Plate
350μL, 1mL, 2mL
Availability: In Stock
Protein Precipitation Plate
Description
96-Well Protein Precipitation Plate for High-Throughput Sample Preparation and LC-MS Analysis
Designed for high-throughput removal of proteins from plasma and serum samples prior to LC-MS analysis, this 96-well protein precipitation plate enables consistent and scalable sample preparation in bioanalytical and pharmacokinetic workflows.
As a standardized PPT plate (protein precipitation plate), it supports parallel processing of multiple samples while maintaining reproducible protein removal and clean extract recovery required for analytical accuracy.
It is widely used in bioanalysis, pharmacokinetics, drug discovery, and LC-MS sample preparation workflows.
Selection Rules for Protein Precipitation Plates
Plasma or serum sample preparation
Protein precipitation plates are recommended over standard filter plates to avoid clogging and ensure consistent protein removal.
LC-MS or LC-MS/MS workflows
Protein removal plate formats are preferred to reduce matrix interference and improve analytical reproducibility.
High-throughput laboratories
96-well PPT plate format is required for parallel processing and automation compatibility.
Low protein carryover requirement
Precipitation-based cleanup is more reliable than direct filtration for biological matrices.
Typical Applications and Recommended Use
| Application Area | Recommended Workflow | Key Requirement |
| Bioanalytical sample prep | Organic solvent precipitation + plate-based separation | LC-MS / LC-MS/MS ready samples |
| Pharmacokinetics & drug metabolism | High-throughput protein removal | Reproducible recovery |
| Clinical & research labs | Standardized multi-sample preparation | Sample cleanup before analysis |
| High-throughput screening | Parallel processing of large sample sets | 96-well compatibility |
Product Function in Workflow
The protein precipitation plate is used in workflows where protein interference must be removed before analytical detection.
Typical process:
- Add organic solvent such as acetonitrile or methanol to biological sample
- Proteins denature, precipitate, and aggregate
- Supernatant passes through the plate under vacuum or centrifugation
- Clean filtrate is collected for downstream analysis
Protein precipitation using organic solvents followed by plate-based separation is a widely adopted standard approach in LC-MS sample preparation workflows.
This process enables efficient removal of protein matrices that may interfere with chromatographic or mass spectrometric analysis.
Distinction from Standard 96-Well Filter Plates
A protein precipitation plate (PPT plate) combines solvent-based protein denaturation with subsequent filtration, allowing efficient separation of precipitated proteins from the liquid phase.
In contrast, a standard 96-well filter plate relies only on membrane pore size for physical retention and is designed for general clarification rather than protein removal.
Using a standard filter plate for protein precipitation often results in membrane clogging, inconsistent flow, and reduced sample recovery.
Plate Design and Structure
The plate is designed for compatibility with standard laboratory automation and high-throughput workflows.
Configuration includes:
- 96-well plate format for parallel processing
- Uniform well structure for consistent flow
- Compatible with vacuum manifolds and centrifugation systems
- Designed for integration with collection plates or deep-well plates
This structure ensures reproducible sample handling across large sample batches.
Technical Specifications
| Parameter | Specification |
| Plate format | 96-well |
| Well volume options | 350 µL / 1 mL / 2 mL |
| Working volume (recommended) | 50–250 µL (350 μL plate) / 100–800 μL (1 mL plate) / 200–1800 μL (2 mL plate) |
| Pore size range | 0.2 – 0.45 μm |
| Filter membrane material | Glass fiber / Polyethylene (PE) |
| Plate material | Polypropylene (PP) |
| Plate dimensions | Standard microplate (approx. 127.8 x 85.5 x 30.6 mm) |
| Processing methods | Vacuum filtration / Centrifugation |
| Typical samples | Plasma, serum, biological fluids |
| Workflow type | High-throughput sample preparation |
| Automation compatibility | Compatible with automated liquid handling systems |
Key Advantages for Laboratory Use
High-throughput processing enables simultaneous preparation of 96 samples
Consistent protein removal improves batch-to-batch reproducibility
Compatible with both vacuum and centrifugation workflows
Reduced manual handling compared with tube-based precipitation
Improved analytical performance by reducing protein interference prior to LC-MS analysis
Comparison with Traditional Protein Precipitation Methods
| Parameter | Plate-Based Method | Tube-Based Method |
| Throughput | High (96 samples) | Low (1 sample at a time) |
| Reproducibility | High (standardized) | Operator dependent |
| Workflow speed | Fast | Slower |
| Automation compatibility | High | Limited |
| Sample handling | Reduced manual steps | Multiple manual transfers |
System Compatibility
Compatible with:
- Vacuum manifold systems
- Centrifuge-based plate processing
- Standard 96-well collection plates
- Automated liquid handling systems
Ordering Information
| Cat. No. | Well Volume | Pore Size | Membrane | Qty/Pk | Pk/Carton |
| 25012001 | 350 μL | 0.2–0.45 μm | Glass fiber / PE | 2 pcs | 25 pk |
| 25012002 | 1 mL | 0.2–0.45 μm | Glass fiber / PE | 2 pcs | 25 pk |
| 25012003 | 2 mL | 0.2–0.45 μm | Glass fiber / PE | 2 pcs | 25 pk |
Bulk supply, OEM customization including plate color, packaging, and barcode labeling are available upon request.
Procurement FAQ
What is a protein precipitation plate used for?
It is used to remove proteins from biological samples such as plasma and serum prior to LC-MS or LC-MS/MS analysis
Can it be used with vacuum or centrifugation?
Yes, it supports both vacuum manifold and centrifuge processing
What sample volumes can be processed?
Available in 350 µL, 1 mL, and 2 mL well volumes with defined working ranges
Why use a plate instead of tubes?
Higher throughput, better reproducibility, and reduced manual handling
Is it suitable for LC-MS workflows?
Yes, it is widely used in bioanalytical and pharmacokinetic workflows
How is this different from a normal filter plate?
This PPT plate uses solvent-based protein precipitation followed by filtration, while standard filter plates rely only on pore size and are not optimized for protein removal
Do you provide certificates of analysis?
Yes, available upon request for each production batch
Request a Quote or Technical Support
For bulk procurement, OEM projects, or application-specific recommendations, technical support is available.
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