· Stationary phase: Phenyl
· Particle size: 3μm / 5μm / 10μm
· Pore size: 120Å / 300Å
· Column dimensions: Internal diameter × Length
· Quantity requirement
Availability: In Stock
Phenyl HPLC Column
Description
Hawach Phenyl HPLC Column is a reversed-phase chromatography column designed with phenyl bonded silica stationary phase to provide unique aromatic selectivity and enhanced retention for compounds containing aromatic rings, π-electron systems, and hydrophobic functional groups.
Compared with conventional C18 columns, Phenyl HPLC Columns provide different molecular interaction mechanisms through π-π interactions, hydrophobic interactions, and aromatic selectivity, making them valuable for separation challenges where traditional alkyl phases show insufficient selectivity.
Key Features
- Unique Phenyl Selectivity -provides chromatographic selectivity different from C18 and C8, improving resolution of structurally similar aromatic compounds
- Excellent Peak Shape -optimized silica surface treatment reduces secondary interactions
- High-Purity Silica Matrix -controlled pore structure and surface modification technology
- Reliable Reproducibility -stable retention time, consistent selectivity, low column-to-column variation
- Wide Range of Dimensions -1mm to 7.8mm ID, 30mm to 300mm length
- Broad Compatibility -works with HPLC, UHPLC and LC-MS systems
Technical Highlights
| Parameter | Specification |
| Stationary Phase | Phenyl bonded silica |
| Mode | Reversed-phase HPLC |
| Particle Sizes | 3μm / 5μm / 10μm |
| Pore Size | 120Å (300Å option available) |
| Endcapping | Available |
| pH Range | Compatible with typical reversed-phase conditions |
Phenyl vs C18 vs C8 – Quick Comparison
| Feature | Phenyl Column | C18 Column | C8 Column |
| Stationary Phase | Phenyl bonded silica | Octadecyl bonded silica | Octyl bonded silica |
| Main Interaction | Hydrophobic + π-π | Hydrophobic | Hydrophobic |
| Aromatic Selectivity | Excellent | Good | Moderate |
| Isomer Separation | Often improved | Sometimes limited | Limited |
| Best For | Aromatic drugs, phenols, PAHs, natural products | General pharmaceutical analysis | General reversed-phase separation |
Why Choose Phenyl HPLC Column?
- π-π interaction with aromatic molecules
- Enhanced positional isomer discrimination
- Different elution order compared with alkyl phases
- Improved resolution for aromatic analytes
- Effective complementary option when C18 separation lacks selectivity
Applications
| Application Area | Typical Use |
| Pharmaceutical Analysis | APIs, drug impurities, related substances, stability testing, intermediates |
| Chemical Analysis | Aromatic compounds, organic synthesis products, reaction monitoring |
| Natural Products | Flavonoids, phenolic compounds, plant extracts, bioactive molecules |
| Environmental Analysis | Aromatic pollutants, organic contaminants, PAHs |
Particle Size Selection
| Particle Size | Best For | Key Advantages |
| 3μm | High-resolution analysis, UHPLC, LC-MS | Higher efficiency, faster analysis, sharper peaks |
| 5μm | Routine analytical HPLC, QC labs | Balanced pressure and efficiency, excellent reproducibility |
| 10μm | Preparative chromatography, purification | Lower back pressure, higher loading capacity |
Ordering Information – Selected SKUs
Phenyl, 3μm, 120Å – Representative Dimensions
| Cat# | Description |
| 13011540 | Phenyl, 3μm, 120Å, 2.1×50mm |
| 13011542 | Phenyl, 3μm, 120Å, 2.1×100mm |
| 13011544 | Phenyl, 3μm, 120Å, 2.1×150mm |
| 13011546 | Phenyl, 3μm, 120Å, 2.1×250mm |
| 13011552 | Phenyl, 3μm, 120Å, 3.0×150mm |
| 13011560 | Phenyl, 3μm, 120Å, 3.9×150mm |
| 13011563 | Phenyl, 3μm, 120Å, 3.9×250mm |
| 13011571 | Phenyl, 3μm, 120Å, 4.6×150mm |
| 13011573 | Phenyl, 3μm, 120Å, 4.6×250mm |
Phenyl, 5μm, 120Å – Representative Dimensions
| Cat# | Description |
| 13011576 | Phenyl, 5μm, 120Å, 2.1×50mm |
| 13011578 | Phenyl, 5μm, 120Å, 2.1×100mm |
| 13011580 | Phenyl, 5μm, 120Å, 2.1×150mm |
| 13011582 | Phenyl, 5μm, 120Å, 2.1×250mm |
| 13011588 | Phenyl, 5μm, 120Å, 3.0×150mm |
| 13011596 | Phenyl, 5μm, 120Å, 3.9×150mm |
| 13011599 | Phenyl, 5μm, 120Å, 3.9×250mm |
| 13011609 | Phenyl, 5μm, 120Å, 4.6×150mm |
| 13011611 | Phenyl, 5μm, 120Å, 4.6×250mm |
| 13011620 | Phenyl, 5μm, 300Å, 4.6×250mm (300Å option) |
Phenyl, 10μm, 120Å – Representative Dimensions
| Cat# | Description |
| 13011623 | Phenyl, 10μm, 120Å, 4.6×150mm |
| 13011625 | Phenyl, 10μm, 120Å, 4.6×250mm |
| 13011626 | Phenyl, 10μm, 120Å, 4.6×300mm |
Full SKU list available – including all 2.1mm, 3.0mm, 4.0mm, 4.6mm, 6.0mm, 6.5mm, 7.8mm ID dimensions. Contact info@hawach.com for complete catalog.
Or download the PDF below.
Download PDF Datasheet
Recommended Operating Conditions
| Parameter | Typical Range |
| Mode | Reversed-phase HPLC |
| Mobile Phase | Water/acetonitrile, water/methanol, buffered aqueous phases |
| Common Additives | Formic acid, acetic acid, ammonium acetate, ammonium formate |
| Temperature | Ambient to elevated temperature |
| Detection | UV, PDA, MS, LC-MS/MS |
Quality Control
- Each Hawach Phenyl HPLC Column undergoes strict quality testing before shipment:
- Column Efficiency -theoretical plate number, peak symmetry, retention performance
- Reproducibility -retention time consistency, selectivity stability, batch performance
- Mechanical Testing -pressure resistance, hardware sealing, connection reliability
FAQs
What is a Phenyl HPLC Column?
A reversed-phase column with phenyl bonded silica stationary phase. It provides unique aromatic selectivity through π-π interactions and hydrophobic interactions.
What is the difference between Phenyl and C18?
C18 relies mainly on hydrophobic retention, while Phenyl provides additional π-π interaction for aromatic compounds. Phenyl phases offer different selectivity and improved resolution for aromatic analytes.
What compounds can be separated using Phenyl columns?
Pharmaceutical compounds, aromatic drugs, phenols, flavonoids, steroids, PAHs, and organic synthesis intermediates.
Can Phenyl columns be used for LC-MS?
Yes, with compatible mobile phases such as water/acetonitrile, water/methanol, formic acid, and ammonium formate buffers.
What particle sizes are available?
3μm (high-efficiency), 5μm (routine HPLC), and 10μm (preparative).
What pore sizes are available?
Standard 120Å, with a 300Å option available (Cat# 13011620 – Phenyl, 5μm, 300Å, 4.6×250mm).
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