Standard Large-Volume Centrifuge Bottle Compatible with Nalgene, Thermo & Corning Rotors.
Availability: In Stock
250 mL Centrifuge Bottles – 6,000 ×g, Sterilized, Non-Pyrogenic
Description
Hawach 250 mL centrifuge bottles are designed for large-volume centrifugation, bulk biological sample processing, cell harvesting, microbial precipitation and routine bioprocessing workflows. Manufactured from high-purity virgin polypropylene in standardized cleanroom environments, these bottles deliver leak-resistant sealing, reinforced wall stability and consistent non-pyrogenic grade performance.
Validated up to 6,000 ×g under standard rotor-supported conditions, the bottles match mainstream large-volume rotor dimensions, requiring no adapters or workflow adjustments.
Quick Technical Summary
| Item | Specification |
| Product Type | 250 mL large-volume centrifuge bottle |
| Bottle Style | Standard conical design |
| Maximum Validated RCF | 6,000 ×g |
| Material | High-purity virgin polypropylene |
| Cap Type | Leak-resistant screw cap |
| Sterility Grade | Factory sterilized |
| Biological Grade | Non-Pyrogenic |
| Temperature Resistance | -80°C to +121°C |
| Typical Applications | Cell harvesting, microbial collection, liquid separation, bioprocessing |
Decision Table
| Problem | Solution in Hawach Bottle | Outcome | Impact |
| Fluid leakage at 6,000 ×g | Leak-resistant screw cap, optimized thread seal | Minimized sample loss, zero rotor contamination | High (critical for bioprocessing safety) |
| Bottle deformation under full load | Reinforced integrated wall structure | Stable shape, consistent pelleting across batches | High (cost saving, reduced reruns) |
| Pyrogen residue interfering with cell culture | Non-pyrogenic manufacturing & screening | Reliable cell assay data, no pyrogen-related inhibition | High (regulatory compliance) |
| Temperature cycling failure (freeze/autoclave) | -80°C to 121°C validated tolerance | One bottle handles storage, sterilization, and centrifugation | Medium (workflow simplification) |
| Chemical incompatibility (solvent swelling) | High-purity PP, broad chemical resistance | No material degradation, safe with alcohols & weak acids | Medium (operational reliability) |
Common Problems in Large-Volume Centrifugation
Laboratories running large-volume workflows often encounter:
- Bottle leakage during medium-speed high-load runs
- Structural deformation under repeated 6,000 ×g cycles
- Pyrogen residue interfering with cell culture assays
- Sample degradation due to improper temperature tolerance
- Chemical incompatibility causing material swelling
- Batch inconsistency affecting reproducibility
Hawach 250 mL centrifuge bottles are engineered to mitigate all these risks.
Rotor Compatibility Matrix
| Rotor Type | Compatibility |
| Swing Bucket Rotor | Fully compatible |
| Fixed Angle Rotor | Fully compatible |
| 250 mL Standard Cavities | Dimension match |
Major brand rotor compatibility – Beckman, Thermo Scientific, Eppendorf, Sorvall, Hettich, VWR.
No adapter or rotor parameter adjustment required.
Compatible Replacement For
Hawach 250 mL centrifuge bottles adopt industry-standard dimensional and structural design, serving as an equivalent, rotor-compatible replacement for:
- Nalgene large-volume centrifuge bottles
- Thermo Scientific 250 mL centrifuge bottles
- Corning large-volume centrifuge containers
Seamless switching – no workflow modification needed.
Temperature Resistance Performance
| Temperature Condition | Performance |
| Ultra-low freezer (-80°C) | Stable, no cracking |
| Standard autoclaving (121°C) | Compatible, 20 min cycles |
| Freeze-thaw cycling | Supported for routine lab use |
| Recommended operating range | -20°C to 50°C (optimal) |
Chemical Compatibility Profile
Compatible With
- Aqueous laboratory buffers (PBS, Tris, etc.)
- Biological cell culture media & supernatants
- Alcohols (ethanol, methanol, isopropanol)
- Weak acids and weak bases (acetic acid, ammonia)
- Fermentation and microbial liquids
Recommended To Avoid
- Strong oxidizing solvents (concentrated H₂SO₄, HNO₃)
- Aromatic hydrocarbons (benzene, toluene)
- Halogenated organic solvents (chloroform, dichloromethane)
Performance Benchmark
Test conditions follow ISO-like centrifugation guidelines for large-volume rotors (6,000 ×g, rated fill volume, room temperature, standard biological buffer). Internal validation protocol for lab-grade benchmark definition.*
| Performance Metric | Generic Large-Volume Bottles | Hawach 250 mL Bottles |
| Leakage under rated RCF | Occasional occurrence | Significantly reduced |
| Structural stability | Deformation tendency under full load | Stable, no bulging |
| Biological purity | Uncontrolled pyrogen level | Consistent non-pyrogenic |
| Temperature cycle tolerance | Material aging after repeated autoclaving | Stable physical property retention |
| Batch consistency | Variable quality | Stable lot-to-lot traceable |
Technical Specifications
| Attribute | Specification |
| SKU Number | 11351001 |
| Nominal Volume | 250 mL |
| Product Name | 250 mL Centrifuge Bottle |
| Biological Grade | Non-Pyrogenic |
| Sterility | Factory sterilized (E-beam) |
| Maximum Validated RCF | 6,000 ×g |
| Raw Material | High-purity virgin polypropylene |
| Cap Design | Leak-resistant screw cap |
| Temperature Range | -80°C to +121°C |
| Rotor Fit | Swing bucket / fixed angle universal |
Related Centrifuge Bottle Sizes
| Volume | Max RCF | Sterility | Application |
| 50 mL centrifuge tubes | 12,000 ×g | Sterile | routine molecular biology |
| 250 mL centrifuge bottles | 6,000 ×g | Sterile | bioprocessing |
| 500 mL centrifuge bottles | 6,000 ×g | Sterile | large-volume harvesting |
Outcome-Driven Laboratory Applications
Biotech & Cell Culture Laboratories
Used for: large-volume cell supernatant separation, suspension cell harvesting, medium concentration.
– Outcome: Reduced leakage and contamination risk during repeated harvesting cycles, improving cell viability data consistency.
Microbiology & Fermentation Labs
Used for: bacterial/yeast strain collection, fermentation liquid clarification, large-scale microbial precipitation.
– Outcome: Stable structural performance across batches, minimizing workflow interruptions and reruns.
Pharmaceutical & Bioprocessing
Used for: raw material separation, intermediate liquid clarification, QC sample preparation.
– Outcome: Consistent batch quality fits GLP/GMP laboratory management, lowering compliance risk.
Research Institutes & Industrial Labs
Used for: large-volume routine centrifugation, bulk sample low-temperature archiving, general bioprocess experiments.
– Outcome: Reliable daily operation with less time spent on consumable failure troubleshooting.
Manufacturing & Supply Stability
- ISO cleanroom production -standardized environment, automated injection molding
- Full lot traceability -each batch documented for quality audit
- Stable raw material supply -long-term order continuity guaranteed
- OEM/private label capacity -custom printing, packaging, barcode labeling
- Export-ready packaging -standard cartons suitable for sea/air shipment
Packaging Options
| Catalog Number | Description | Packaging |
| 11351001 | 250 mL Centrifuge Bottle, Sterilized, Non-Pyrogenic | 4 pcs/bag, 25 bags/carton (100 pcs/carton total) |
OEM & Bulk Supply Support
Available services:
- Custom logo printing (MOQ available on request)
- Private labeling and tailored packaging
- Distributor reserved stocking programs
- Full lot traceability documentation
- COA, sterility, and non-pyrogenic certificates provided
- Long-term fixed-price agreements for institutional and distributor partners
Frequently Asked Questions
Technical FAQs
Are these 250 mL centrifuge bottles sterilized?
Yes, factory sterilized (E-beam) and ready for direct laboratory use.
What is the maximum centrifugal force rating?
Validated for stable performance up to 6,000 ×g under standard rotor-supported conditions.
Are the bottles non-pyrogenic?
Yes, all production batches maintain consistent non-pyrogenic grade.
Can I autoclave them? Are they freezer safe?
Yes. Stable at -80°C long-term storage and compatible with 121°C autoclaving (20 min cycles).
Which rotors and brands are compatible?
Fits swing bucket and fixed angle 250 mL standard cavities. Compatible with Beckman, Thermo, Eppendorf, Sorvall, Hettich, VWR.
Can it replace Nalgene, Thermo, or Corning 250 mL bottles?
Yes. Standard dimensions and rotor-friendly design allow direct workflow replacement without equipment adjustment.
Procurement & Distributor FAQs
Do you support annual price locking for long – term contracts?
Yes, for institutional labs, biopharma enterprises, and regular distributor partners.
Can you provide distributor reserved inventory programs?
Yes, reserved stock allocation and phased delivery schedules available.
Are pallet, weight, and container loading specs available?
Yes, full shipping data provided for bulk ocean shipments.
Can you support custom barcode labeling and private branding?
Yes, for qualified bulk and OEM orders.
Do you allow split shipments or regional warehouse delivery?
Flexible arrangements for long-term cooperative clients.
Request Samples or Bulk Quotation
Request free evaluation samples, rotor compatibility confirmation, bulk pricing, OEM customization, QA documentation, or distributor partnership details.
Submit your annual consumption volume and packaging preference – you will receive a customized quotation within 24 hours.
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