Conical Culture Flask | Sterile Shake Flasks for Cell Culture

Conical culture flasks are sterile laboratory vessels used for suspension cell culture, microbial growth, and medium preparation. Also known as Erlenmeyer shake flasks, they are designed to improve oxygen transfer, reduce contamination risk, and ensure stable cell growth in shaking incubators.

Hawach supplies DNase/RNase-free conical culture flasks in 125mL–1000mL, available with vented or sealed caps, supporting biotechnology, pharmaceutical, and microbiology applications worldwide.

What Is a Conical Culture Flask?

A conical culture flask is a narrow-neck, wide-bottom laboratory vessel specifically designed for:

• Efficient oxygen exchange during shaking culture
• Reduced contamination risk through controlled opening
• Uniform mixing without liquid spillage
• Stable suspension of cells and microorganisms

Typical Uses

• Suspension cell culture (CHO, HEK293, Jurkat)
• Microbial culture (bacteria, yeast, fungi)
• Culture media preparation and storage
• Biochemical and fermentation reactions

Key Features of Hawach Conical Culture Flask

High-Purity Medical Grade Materials

Manufactured from PETG or polycarbonate (PC):

• High transparency for visual monitoring of cell growth
• Non-cytotoxic and biologically inert, no interference with cell growth
• Chemically resistant and durable for laboratory use

Sterile & Contamination-Free

• Gamma sterilized (Sterility Assurance Level, SAL 10⁻⁶)
• DNase/RNase-free
• Endotoxin level < 0.1 EU/mL (USP standard)
• Individually sterile packed for clean handling

Optimized for Suspension Cell Growth

• Ultra-smooth inner surface minimizes cell adhesion, ideal for suspension culture
• Supports high-density cell culture and consistent growth
• Ensures reproducible experimental conditions and reliable results

Vented & Sealed Cap Options

• Vented caps: Equipped with gas exchange membrane for aerobic culture, ensuring optimal oxygen supply
• Sealed caps: Leak-proof design, ideal for transport, storage, and low-evaporation requirements

Wide Temperature Compatibility

• Operating range: -80°C to +121°C
• Compatible with standard laboratory incubators, shakers, and freezers

Leak-Proof One-Piece Design

• Manufactured with integrated blow-molding technology
• No weak sealing points or joints
• Stable performance during high-speed shaking and handling

Product Specifications

Download PDF Datasheet

Applications

Cell Culture

• Stem cell expansion and research
• Insect and plant cell culture
• Mammalian suspension cells (CHO, HEK293), commonly used with cell culture flasks and cell culture plates

Microbiology & Fermentation

• Bacterial and yeast cultivation
• Fungal growth studies
• Fermentation process development

Biotechnology & Pharma

• Vaccine development and viral culture
• Recombinant protein expression and purification
• Drug screening and cytotoxicity testing

Laboratory Use

• Buffer and reagent preparation
• Media storage and transport
• Sterile mixing applications

Conical Flask vs Other Culture Vessels

How to Use a Conical Culture Flask (Best Practice)

• Fill volume at 20–40% of total capacity for optimal aeration and mixing
• Use vented caps for aerobic cell culture to ensure gas exchange
• Maintain shaking speed between 100–250 rpm, depending on cell type
• Avoid overfilling to ensure proper oxygen transfer and prevent spillage

Why Choose Hawach?

• Stable bulk production capacity for global supply
• OEM & private label customization (branding, packaging, specifications)
• Strict batch traceability system for regulatory compliance
• Global supply for distributors, laboratories, and biotech companies

Packaging & Quality Control

• Individually sterile PE packaging for clean handling
• Lot traceable for compliance with industry standards
• Leak-proof and sterility tested before delivery
• 36-month shelf life under proper storage (15–25°C, away from direct sunlight and moisture)

Frequently Asked Questions (FAQ)

What is a conical culture flask used for?

It is used for suspension cell culture, microbial growth, media preparation, and biochemical reactions in laboratories and industrial research.

What size conical flask should I choose?

The working volume should be 20–40% of total capacity, ensuring proper oxygen transfer and mixing efficiency. Choose the volume based on your experimental scale.

What is the difference between PETG and PC flasks?

PETG is lightweight and cost-effective with high clarity, while PC offers higher strength and temperature resistance for demanding applications (e.g., high-intensity shaking).

Should I use vented or sealed caps?

Use vented caps for cell culture requiring gas exchange (aerobic culture), and sealed caps for storage, transport, or low-evaporation requirements.

Are the flasks sterile and safe for cell culture?

Yes, they are gamma sterilized (SAL 10⁻⁶), DNase/RNase-free, endotoxin-free, and non-cytotoxic, ensuring no interference with cell growth and experimental results.

Do you support OEM and bulk orders?

Yes, Hawach provides OEM customization and large-volume supply for distributors and laboratories worldwide, with flexible MOQ and global logistics support.

Order informaiton

Picture	Cat#	Description	Packaging(pcs/pk)
	16092101	125mL Conical Culture Flask, Vent Cap, PC, 1pc*50bags/Carton	50
	16092102	250mL Conical Culture Flask, Vent Cap, PC, 1pc*50bags/Carton	50
	16092103	500mL Conical Culture Flask, Vent Cap, PC, 1pc*25bags/Carton	25
	16092201	1000mL Conical Culture Flask, Vent Cap, PETG, 1pc*25bags/Carton	25

Order Conical Culture Flasks from Hawach

Looking for a reliable conical culture flask manufacturer?

• Consistent bulk supply with strict quality control
• OEM customization (branding, packaging, specifications)
• Fast global delivery for distributors and laboratories

Contact Hawach today to request samples, technical datasheets, or a quotation.