Large-Volume Bioprocessing Flask for High-Throughput Cell Harvesting & Industrial Fermentation
Availability: In Stock
500 mL Centrifuge Bottles – 6,000 ×g, Sterilized, Non-Pyrogenic
Description
For laboratories scaling from benchtop to production, Hawach 500 mL centrifuge bottles close the gap between small-volume R&D and industrial bioprocessing. Manufactured from high-purity virgin polypropylene in ISO cleanroom environments, these bottles are designed for high-throughput cell harvesting, large-scale microbial precipitation, and upstream bioprocess sample concentration.
Validated to 6,000 ×g under full rotor support, the 500 mL format fits standard large-volume centrifuge rotors from leading brands. Each bottle is factory sterilized and certified non-pyrogenic, ready for direct use in regulated biopharma workflows.
Unlike smaller 250 mL bottles, the 500 mL version is optimized for:
- Batch volume parity with shake flasks and bioreactor harvests
- Reduced number of centrifuge runs per total sample volume
- Stackable storage and freezer organization
- Direct scaling from pilot to production without protocol changes
Quick Technical Summary
| Item | Specification |
| SKU Number | 11351002 |
| Nominal Volume | 500 mL |
| Product Type | Large-volume centrifuge flask / bottle |
| Maximum Validated RCF | 6,000 ×g |
| Material | High-purity virgin polypropylene |
| Cap Type | Leak-resistant screw cap |
| Sterility Grade | Factory sterilized (E-beam) |
| Biological Grade | Non-Pyrogenic |
| Temperature Resistance | -80°C to +121°C |
| Packaging | 2 pcs/bag, 20 bags/carton (40 pcs/carton total) |
| Typical Applications | Bulk cell harvesting, fermentation concentration, large-scale protein precipitation, bioprocess QC |
Decision Table – Problem – Solution – Outcome – Impact
| Problem | Solution in Hawach 500 mL Bottle | Outcome | Impact |
| Too many centrifuge runs per harvest batch | 500 mL single-bottle capacity | 2.5× fewer runs compared to 200 mL bottles | High (time & energy saving at scale) |
| Bottle deformation under full load at 6,000 ×g | Reinforced wall geometry for 500 mL volume | Stable structure, no bulging, consistent pellet formation | High (reproducibility in production) |
| Cross-batch pyrogen variability | Non-pyrogenic grade with lot-specific testing | Meets biopharma QC release criteria | High (regulatory compliance) |
| Bottles not stackable for freezer storage | Uniform external geometry with flat top | Stackable 3-4 high, saves freezer space | Medium (laboratory organization) |
| Scaling from R&D to production requires new validation | Same material, same RCF rating as 250 mL version | Direct scale-up – no revalidation needed | High (seamless process transfer) |
Common Problems in High-Volume Bioprocessing
Industrial and high-throughput labs face unique challenges when moving beyond 250 mL:
- Run count explosion -Processing a 10 L bioreactor harvest with 250 mL bottles requires 40 runs; with 500 mL bottles, only 20 runs.
- Pellet instability -Larger volume creates higher hydraulic pressure during deceleration, causing pellet resuspension if tube geometry is not optimized.
- Sterility breaches -More runs equal more cap handling, increasing contamination probability.
- Cold storage inefficiency -Non-stackable bottles waste valuable -80°C footprint.
- Scale-up friction -Different bottle dimensions or RCF ratings between R&D and production force protocol revalidation.
Hawach 500 mL centrifuge bottles are engineered specifically to eliminate these scaling frictions.
Rotor Compatibility Matrix (500 mL Specific)
| Rotor Type | Compatibility | Notes |
| Swing Bucket Rotor (500 mL buckets) | Fully compatible | Standard bucket depth accepts 500 mL bottle height |
| Fixed Angle Rotor (500 mL cavities) | Fully compatible | 45° angle typical; no adapter required |
| 250 mL Rotors | Not compatible | Physical dimensions differ – do not force fit |
Major brand rotor systems validated:
- Beckman Coulter (JLA, J series large-volume rotors)
- Thermo Scientific (Fiberlite, SLA rotors)
- Eppendorf / Sorvall (SLC, F9 series)
- Hettich (RotoSilenta, Universal series)
- VWR (standard 500 mL rotor buckets)
Always confirm rotor model. Free rotor compatibility verification available upon request.
Compatible Replacement For
Hawach 500 mL centrifuge bottles are dimensionally and functionally equivalent to:
- Nalgene 500 mL centrifuge bottles (polypropylene, screw cap)
- Thermo Scientific Nunc 500 mL centrifuge bottles
- Corning 500 mL centrifuge flasks
Seamless drop-in replacement – no rotor adjustment, no cap adapter, no workflow retraining.
Temperature Resistance Performance
| Temperature Condition | Performance |
| Ultra-low freezer (-80°C) | Stable, no cracking; supports long-term biobanking |
| Standard autoclaving (121°C, 20 min) | Fully compatible |
| Freeze-thaw cycling (x10 cycles) | No loss of mechanical integrity |
| Working temperature range | +4°C (cold room) to +50°C (warm buffers) |
Note: For critical high-speed centrifugation, single-use after autoclaving recommended.
Chemical Compatibility Profile
Compatible With (typical bioprocess reagents)
- Cell culture media (DMEM, RPMI, Schneider‘s)
- Bacterial fermentation broths (LB, TB, SB)
- Yeast peptone dextrose (YPD) media
- Phosphate buffers (PBS, PBST)
- Alcohols (20% ethanol, isopropanol for cleaning)
- Diluted acids/bases (0.1N HCl, 0.1N NaOH)
Not Recommended For
- Concentrated strong acids (>1N) or bases (>1N)
- Organic solvents >20% (acetonitrile, chloroform)
- Phenol, chloroform extraction mixtures
For aggressive solvent applications, contact technical support for material upgrade options.
Performance Benchmark
Test conditions follow ISO-like centrifugation guidelines for large-volume bioprocessing rotors (6,000 ×g, 500 mL rated fill, room temperature, standard biological broth). Internal validation protocol for industrial-grade benchmark definition.*
| Performance Metric | Generic 500 mL Bottles | Hawach 500 mL Bottles |
| Leakage at 6,000 ×g | Occasional visible wetness | No leakage observed under test |
| Wall deformation at full load | Bulging visible in 20% of runs | No deformation |
| Stackability for -80°C storage | Not stackable (rounded top) | Stackable (flat-top design) |
| Bioburden after sterilization | Variable (0.1-1% positive) | Consistent sterility SAL 10⁻⁶ |
| Batch-to-batch dimensional tolerance | ±2% variation | ±0.5% (injection molding control) |
Technical Specifications
| Attribute | Specification |
| SKU Number | 11351002 |
| Nominal Volume | 500 mL |
| Product Name | 500 mL Centrifuge Flask / Bottle |
| Biological Grade | Non-Pyrogenic |
| Sterility | Factory sterilized (E-beam, SAL 10⁻⁶) |
| Maximum Validated RCF | 6,000 ×g |
| Raw Material | High-purity virgin polypropylene |
| Cap Design | Leak-resistant screw cap, 45 mm neck diameter |
| Temperature Range | -80°C to +121°C |
| Outer Diameter (approx.) | 78 mm |
| Height (approx.) | 168 mm |
| Bottle Shape | Cylindrical with conical bottom |
| Graduations | Molded, every 50 mL |
| Writing Area | Frosted panel for labeling |
| Rotor Fit | Swing bucket & fixed angle 500 mL cavities |
| Packaging | 2 pieces/bag, 20 bags/carton (40 pieces total) |
| Carton Dimensions (approx.) | 45 x 35 x 28 cm |
| Gross Weight per Carton | ~6.5 kg (with bottles) |
Related Centrifuge Bottle Sizes
| Volume | Max RCF | Sterility | Application |
| 50 mL centrifuge tubes | 12,000 ×g | Sterile | routine molecular biology |
| 250 mL centrifuge bottles | 6,000 ×g | Sterile | bioprocessing |
| 500 mL centrifuge bottles | 6,000 ×g | Sterile | large-volume harvesting |
Outcome-Driven Laboratory Applications
Bioprocess R&D & Pilot Scale
Used for: Harvesting 5-50 L bioreactor cultures, concentrating fermentation broths, collecting secreted proteins or exosomes.
– Outcome: Reduce centrifuge runs by 50% compared to 250 mL bottles; transfer directly from R&D to production without revalidation.
Cell Culture & Gene Therapy Manufacturing
Used for: Large-volume suspension cell harvesting (CHO, HEK293, Vero cells), lentiviral vector concentration, media clarification.
– Outcome: Maintain non-pyrogenic integrity across hundreds of bottles per batch; stackable design doubles -80°C storage density.
Industrial Microbiology (Food, Pharma, Environmental)
Used for: Biomass collection from 20 L to 200 L fermentation trains, spore harvesting, bacterial cell banking.
– Outcome: Consistent structural performance under repeated high-volume centrifugation; lot traceability meets ISO 9001 audit requirements.
Biopharmaceutical QC & Analytical Labs
Used for: Bulk sample preparation for endotoxin testing, bioburden analysis, host cell protein (HCP) assays.
– Outcome: Pre-sterilized, ready-to-use format reduces QC lab prep time; non-pyrogenic certification supports regulatory filing.
Manufacturing & Supply Stability
- Dedicated 500 mL molding line -automated, high-cavity tooling ensures consistent wall thickness across millions of units
- Lot traceability -each carton labeled with production batch, sterilization date, and expiry (if applicable)
- In-stock inventory -main warehouse holding >50,000 units; regional stock available for key distributors
- Lead time for bulk orders -7-10 working days for sterilized; 3-5 days for non-sterile
- OEM capabilities -custom printing (logo, lot code), private label cartons, barcode labeling
- Export packaging -cartons reinforced for sea freight; pallet specs available on request
Packaging Options
| Catalog Number | Description | Packaging |
| 11351002 | 500 mL Centrifuge Bottle, Sterilized, Non-Pyrogenic | 2 pcs/bag, 20 bags/carton (40 pcs/carton total) |
Why 2 pcs/bag? – Double-bagged format reduces contamination risk when opening a carton; each bag contains a matched pair for paired rotor balancing.
Alternative packaging available upon request:
- Bulk non-sterile (100 pcs/bag, 5 bags/carton) for labs that autoclave in-house
- Racked (if applicable for 500 mL -contact for feasibility)
- Custom bag labeling with your SKU barcode
OEM & Bulk Supply Support
Available services for high-volume buyers:
| Service | Availability |
| Custom logo printing (bottle side) | MOQ 5,000 pieces per design |
| Private label carton | MOQ 1,000 cartons |
| Barcode labeling (custom SKU) | MOQ 10,000 pieces |
| Distributor reserved stocking | Negotiated annually |
| Lot-specific COA & sterility certificates | Provided for every batch |
| Long-term fixed pricing (1-3 years) | For annual volumes ≥20,000 pieces |
Frequently Asked Questions
Technical FAQs (500 mL Specific)
Q: What makes the 500 mL bottle different from the 250 mL version?
A: The 500 mL bottle has a larger diameter and taller height, requiring specific 500 mL rotor buckets. It is not a scaled-up 250 mL shape but a separate industrial format.
Q: Can I use this 500 mL bottle in a 250 mL rotor bucket?
A: No. The dimensions are incompatible. Always use 500 mL-rated rotors.
Q: Is the 500 mL bottle really validated to 6,000 ×g at full 500 mL fill?
A: Yes, under standard conditions with supported rotor buckets. For fixed-angle rotors, we recommend ≤450 mL fill to account for meniscus effect.
Q: Are these bottles RNase/DNase-free in addition to non-pyrogenic?
A: Yes, each batch is tested for nuclease-free and endotoxin-free. COA available.
Q: Can the bottles be centrifuged again after freezing?
A: Yes, but inspect for cracks after thawing. Single freeze-thaw cycle is fine; repeated cycles may weaken polypropylene.
Q: How do I properly seal the cap for high-speed runs?
A: Screw until finger-tight, then an additional quarter-turn. Do not over-tighten (can crack threads).
Procurement & Distributor FAQs (500 mL Bulk Focus)
Q: What is the minimum order quantity for stock (non-custom) bottles?
A: One carton (40 pieces). Smaller sample packs available upon request.
Q: Do you offer mixed pallets (250 mL + 500 mL bottles)?
A: Yes, for distributor orders. Contact for pallet configuration.
Q: Can you supply bottles without sterilization (non-sterile) for in-house autoclaving?
A: Yes, specify non-sterile when ordering. Lead time shorter (3-5 days).
Q: Is there a bulk discount for annual volumes above 50,000 pieces?
A: Yes, tiered pricing available. Request a volume quote.
Q: Do you provide a certificate of irradiation for E-beam sterilization?
A: Yes, included with each batch. Additional validation reports available upon request.
Request Samples or Bulk Quotation
For process development labs: Request up to 10 free evaluation bottles (sterile, non-pyrogenic) for rotor fit and performance testing.
For procurement teams: Submit your annual volume (pieces or cartons) and packaging preference. Receive tiered pricing, lead time confirmation, and sample COA within 24 hours.
For distributors: Inquire about regional exclusivity, stocking programs, and private label OEM.
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