Glass Bottom Dish Manufacturer | Sterile Imaging Dishes for Cell Culture

Glass bottom dishes are specialized cell culture vessels designed for high-resolution live cell imaging, confocal microscopy, and fluorescence analysis. They combine a plastic culture dish with an ultra-clear glass bottom to provide distortion-free optical performance for advanced laboratory imaging.

A glass bottom dish is a type of imaging cell culture dish with a borosilicate glass base, designed for high-resolution microscopic observation of living or fixed cells.

They are widely used in cell culture consumables, especially in biotechnology, pharmaceutical research, and cell biology laboratories requiring precise imaging and stable cell culture conditions.

What Is a Glass Bottom Dish?

A glass bottom dish is a hybrid laboratory culture vessel consisting of a plastic frame and a thin optical glass base. It is designed to:

• Enable high-resolution microscopic imaging
• Support adherent cell growth and live cell observation
• Minimize optical distortion during fluorescence and confocal imaging
• Maintain a sterile and stable cell culture environment

The glass bottom typically uses 0.16–0.19 mm borosilicate glass, similar to microscope coverslips, ensuring high optical clarity for advanced imaging systems.

Common Applications of Glass Bottom Dishes

Cell Imaging & Microscopy

Glass bottom dishes are primarily used for high-resolution imaging applications, including:

• Live cell time-lapse imaging
• Confocal laser scanning microscopy
• Fluorescence and TIRF imaging
• High-resolution cellular structure analysis

Cell Culture Research

They support advanced biological research such as:

• Adherent cell culture (HEK293, fibroblasts, stem cells)
• Cell migration and invasion studies
• Gene expression and fluorescent labeling
• High-content screening assays

Biomedical & Pharmaceutical Research

Widely used in drug discovery and biomedical studies:

• Drug screening and cytotoxicity testing
• Protein localization and intracellular signaling
• 3D cell culture and tissue engineering models
• Imaging-based diagnostic research

Key Features of Hawach Glass Bottom Dish

Ultra-Transparent Optical Glass Bottom

• Borosilicate glass thickness: 16–0.19 mm
• Coverslip-grade optical clarity for microscopy
• Minimal distortion for confocal and fluorescence imaging

Stable Biocompatible Structure

• Medical-grade adhesive bonding between glass and plastic frame
• High structural stability with no leakage or detachment
• Non-cytotoxic materials suitable for long-term culture

TC-Treated Surface for Cell Adhesion

• Enhances adherent cell attachment and spreading
• Suitable for most mammalian and primary cell lines
• Improves experimental reproducibility

Sterile & Contamination-Free

• Gamma sterilized (SAL 10⁻⁶)
• DNase/RNase-free
• Endotoxin level < 0.1 EU/mL
• Non-cytotoxic and laboratory safe

Optimized for Microscopy Systems

• Compatible with confocal, fluorescence, and TIRF systems
• Standard 35 mm dish format fits most microscope stages
• Stable focal plane for imaging consistency

Product Specifications

Glass Bottom Dish vs Other Cell Culture Vessels

The following comparison helps select the appropriate vessel for different laboratory applications:

Related product: Cell Culture Dish(https://www.hawach.com/cell-culture-consumables/cell-culture-dish.html)

How to Use a Glass Bottom Dish (Best Practice)

• Use TC-treated dishes for adherent cell growth
• Handle carefully to avoid scratching the glass bottom
• Maintain sterile conditions during cell seeding
• Use appropriate CO₂ incubator settings (37°C, 5% CO₂)
• Ensure compatibility with microscope objective working distance

Why Choose Hawach Glass Bottom Dish?

• ISO 13485 certified manufacturing system
• High-precision optical glass integration technology
• Stable bulk production for global supply
• OEM & private label customization support
• Strict batch traceability and QC testing

Packaging & Quality Control

• Sterile packaging (individual or bag pack)
• Lot traceability for regulatory compliance
• QC testing for optical clarity, bonding strength, and sterility
• 36-month shelf life under proper storage conditions (15–25°C)

Frequently Asked Questions (FAQ)

What is a glass bottom dish used for?

Glass bottom dishes are used for live cell imaging, confocal microscopy, fluorescence observation, and high-resolution cellular analysis in research laboratories.

Why use glass bottom instead of plastic dishes?

Glass provides superior optical clarity and minimal distortion, making it essential for high-resolution microscopy compared to standard plastic dishes.

What is the glass thickness used in these dishes?

The glass bottom is typically 0.16–0.19 mm borosilicate glass, equivalent to microscope coverslip quality.

Are glass bottom dishes suitable for cell culture?

Yes, TC-treated surfaces support adherent cell growth while maintaining optical imaging performance.

Do you support OEM and bulk orders?

Yes, Hawach provides OEM customization and bulk supply for global distributors and laboratories.

Order Information

Order Glass Bottom Dishes from Hawach

Looking for a reliable glass bottom dish manufacturer?

Stable optical quality for advanced imaging applications

OEM customization (logo, packaging, specifications)

Fast global delivery for research and industrial clients

Contact Hawach today to request a quote or sample.