Column: XChroma HPLC Column, C18-Alkaline, 4.6 ×250 mm, 5µm
Mobile Phase:
A: 0.5 mol/L KH2PO3(adjust pH to 3.5 with H3PO4): MeOH:water=10:30:60
B: 0.5 mol/L KH2PO3(adjust pH to 3.5 with H3PO4): MeOH:water=10:50:40
Flow Rate: 1.0 mL/min
Detector: 225nm
Temperature: 30°C
Injection Volume: 20μL