RP-C18 / RP-C2 bonded silica stationary phase
Suitable for polar and moderately polar compounds
Available sizes: 5×10, 5×20, 10×20, 20×20 cm
0.25 mm uniform coating for consistent results
Glass-backed for durability and flat surface
Fluorescent / non-fluorescent options available
Ideal for method development and HPLC screening
Availability: In Stock
RP-C18 & RP-C2 Thin Layer Chromatography Plates for Polar Compound Separation
Reversed Phase TLC Plates are thin layer chromatography plates coated with chemically modified silica gel containing hydrophobic alkyl groups such as C18 or C2. Unlike normal-phase TLC plates, reversed phase TLC plates use a non-polar stationary phase and a relatively polar mobile phase, making them ideal for separating polar compounds and samples with complex matrices.
Because reversed phase chromatography follows similar separation principles to reversed phase HPLC, RP-TLC plates are widely used for method development, sample screening, and preliminary chromatographic analysis before HPLC testing.
Hawach Reversed Phase TLC Plates are manufactured using high-purity bonded silica gel and advanced automatic coating technology to ensure uniform coating thickness, excellent reproducibility, and reliable separation performance.
RP-C18 TLC Plates feature octadecyl (C18) bonded silica as the stationary phase and are the most commonly used reversed phase TLC plates. They provide strong hydrophobic interactions and excellent retention for a wide range of pharmaceutical, biochemical, and environmental samples.
RP-C2 TLC Plates utilize ethyl (C2) bonded silica and provide lower hydrophobic retention compared with RP-C18 plates. They are suitable for analytes requiring faster migration and alternative selectivity.
Manufactured using premium RP-C18 and RP-C2 modified silica gel to ensure stable chromatographic performance and reproducible results.
Produced with fully automated coating equipment to achieve highly consistent layer thickness and surface uniformity.
Capable of effectively separating a broad range of polar and moderately polar compounds with high resolution.
Provides chromatographic behavior comparable to reversed phase HPLC, making it a valuable tool for method optimization and preliminary screening.
Available with or without fluorescent indicator to accommodate different visualization requirements.
Durable glass support offers excellent flatness, chemical resistance, and dimensional stability.
Reversed Phase TLC Plates are widely used in:
Compared with traditional silica gel TLC plates, reversed phase TLC plates offer:
Reversed phase TLC and normal phase TLC differ significantly in stationary phase polarity, separation mechanism, and application scope. Understanding these differences helps select the right TLC plate for analytical workflows and method development.
| Feature | Reversed Phase TLC (RP-TLC) | Normal Phase TLC (NP-TLC) |
| Stationary Phase | Hydrophobic bonded silica (C18, C2) | Polar silica gel |
| Separation Mechanism | Hydrophobic interactions | Adsorption polarity |
| Mobile Phase | Polar solvents (water, methanol, buffer) | Non-polar to moderately polar solvents |
| Suitable Compounds | Polar and moderately polar compounds | Non-polar and weakly polar compounds |
| Retention Behavior | More polar compounds retained longer | Less polar compounds retained longer |
| HPLC Correlation | Matches reversed-phase HPLC | Matches normal-phase chromatography |
| Typical Applications | Pharmaceuticals, biomolecules, polar analytes | Lipids, hydrocarbons, non-polar organics |
| Method Development Use | Excellent for RP-HPLC development | Traditional TLC screening |
Conclusion:
RP-TLC Plates are ideal when working with polar compounds or when developing reversed-phase HPLC methods, while NP-TLC Plates are better suited for non-polar compound separation.
| Parameter | Specification |
| Product Type | Reversed Phase TLC Plates |
| Stationary Phase | RP-C18 / RP-C2 Bonded Silica Gel |
| Support Material | Glass |
| Coating Thickness | 0.25 mm |
| Fluorescent Indicator | Available |
| Separation Mode | Reversed Phase Chromatography |
| Application | TLC Analysis and HPLC Method Development |
| Cat. No. | Description |
| 24051001 | RP-C2 TLC Plate, 10 × 20 cm, 0.25 mm, Fluorescent Free, Glass Backed, 25 pcs/pk |
| 24051002 | RP-C2 TLC Plate, 20 × 20 cm, 0.25 mm, Fluorescent Free, Glass Backed, 25 pcs/pk |
| 24051003 | RP-C2 TLC Plate, 10 × 20 cm, 0.25 mm, Fluorescent, Glass Backed, 25 pcs/pk |
| 24051004 | RP-C2 TLC Plate, 20 × 20 cm, 0.25 mm, Fluorescent, Glass Backed, 25 pcs/pk |
| Cat. No. | Description |
| 24051005 | RP-C18 TLC Plate, 20 × 20 cm, 0.25 mm, Fluorescent, Glass Backed, 25 pcs/pk |
| 24051006 | RP-C18 TLC Plate, 10 × 20 cm, 0.25 mm, Fluorescent, Glass Backed, 25 pcs/pk |
| 24051007 | RP-C18 TLC Plate, 10 × 20 cm, 0.25 mm, Fluorescent, Glass Backed, 50 pcs/pk |
| 24051008 | RP-C18 TLC Plate, 5 × 20 cm, 0.25 mm, Fluorescent, Glass Backed, 50 pcs/pk |
| 24051009 | RP-C18 TLC Plate, 5 × 10 cm, 0.25 mm, Fluorescent, Glass Backed, 25 pcs/pk |
RP-C18 plates provide stronger hydrophobic interactions and longer analyte retention, while RP-C2 plates offer weaker retention and faster migration for certain compounds.
Reversed phase TLC is commonly used for polar and moderately polar compounds, including pharmaceuticals, peptides, natural products, pesticides, and biochemical samples.
Yes. Reversed phase TLC is often used for preliminary screening and optimization because its separation mechanism is similar to reversed phase HPLC.
Yes. Hawach offers both fluorescent and non-fluorescent reversed phase TLC plates depending on analytical requirements.
All products are manufactured on high-quality glass-backed plates to ensure excellent flatness and chromatographic consistency.
The quality has a guarantee
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