Column: XChroma HPLC Column, C8-Low PH, 5μm, 4.6×250mm
Mobile Phase: A: 1.0mL TcMB, duplicate in 1000mL water B:acetonitrile
Wavelength: 285nm
Temperature: 40°C
Flow Rate: 1mL / min
Injection Volume: 20μL
Notes: After every injection, need 10 column volume to get gradient equilibrium