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C8 HPLC Columns – Universal, Low pH & Fluorine Phases
Description
Hawach C8 HPLC Columns (octylsilane) are reversed-phase stationary phases with moderate hydrophobicity – less retentive than C18 but more than C4/C2. Designed for LC/MS applications, pharmaceutical, environmental, and bioanalytical method development, they provide excellent peak shape for acidic, basic, and neutral compounds.
Three Specialized C8 Chemistries for Method Development
- C8-Universal: Standard C8, wide pH range (1.5-10), double end-capped, ideal for general method development and LC/MS.
- C8-Low pH: Stable at pH 0.5-8, 100% aqueous mobile phase compatible, perfect for low-pH conditions and highly polar analytes.
- C8-Fluorine: Unique selectivity for halogenated aromatics (F, Cl, Br), orthogonal to conventional C8, for specialized separations.
Why Hawach C8 HPLC Columns Rank for Method Development
- Three distinct selectivities for orthogonal separation
- Wide particle size range: 3, 5, 10 μm; column diameters 2.1-8 mm; lengths 30-300 mm
- 300Å pore size option for large biomolecules
- Double end-capped bonding reduces silanol interactions, ensures sharp peaks
- Compatible with major LC/MS systems: Agilent, Waters, Shimadzu, Thermo
Quick Selection Guide – Choose the Right C8 Phase
| Application | Recommended Phase | Recommended Dimensions |
| General method development, pH 2-8 | C8-Universal | 4.6×150 mm, 5 μm |
| LC/MS (high sensitivity, low flow) | C8-Universal | 2.1×50-100 mm, 3 μm |
| Low pH (<2) or 100% aqueous mobile phase | C8-Low pH | 4.6×150 mm, 5 μm |
| Halogenated pesticides / drug metabolites | C8-Fluorine | 4.6×150 mm, 5 μm |
| Large biomolecules (proteins, peptides) | Any C8 with 300Å pore size | 4.6×250 mm, 5 μm |
| Method development screening | All three phases | 4.6×150 mm, 5 μm each |
Core Technical Specifications
| Parameter | C8-Universal | C8-Low pH | C8-Fluorine |
| Bonded Phase | Octylsilane (C8) | Octylsilane (C8) | Octylsilane + fluorinated modifier |
| Particle Sizes | 3, 5, 10 μm | 3, 5 μm | 3, 5 μm |
| Pore Size | 120Å (300Å option) | 120Å (300Å option) | 120Å |
| Carbon Load | ~12% | ~12% | ~11% |
| End-Capping | Double | Double | Double |
| pH Stability | 1.5-10 | 0.5-8 | 1.5-8 |
| Max Pressure | 4000-6000 psi | 4000-6000 psi | 4000-6000 psi |
| Temperature | ≤60°C | ≤60°C | ≤60°C |
Key Performance Advantages
C8-Universal
- Less retentive than C18 -faster analysis
- Double end-capping -minimizes silanol interactions
- Wide pH range (1.5-10) -compatible with all mobile phases
- LC/MS friendly -reduces run time and solvent use
C8-Low pH
- Extreme low pH tolerance (0.5-8) -use with TFA, formic or phosphoric acid
- 100% aqueous resistant -no phase collapse
- Strong retention for polar compounds -alternative to HILIC
- Same bonding as Universal -consistent reproducibility
C8-Fluorine
- Unique selectivity for halogenated compounds (F, Cl, Br)
- Orthogonal to conventional C8 and C18
- 300Å option -suitable for peptides and small proteins
Typical Applications
| Application | Recommended Phase | Why |
| Pharmaceutical impurity analysis | C8-Universal | Wide pH, excellent peak shape |
| LC/MS bioanalysis (plasma, urine) | C8-Universal 3μm, 2.1 mm | Lower retention, faster run |
| Acidic mobile phase (pH 1.5-2.5) | C8-Low pH | Extended column lifetime |
| 100% buffer mobile phase | C8-Low pH | No phase collapse |
| Halogenated drug metabolites | C8-Fluorine | Enhanced selectivity |
| Method development screening | All three phases | Orthogonal selectivity |
Separation Effect – Typical Chromatograms
C8-Universal
C8-Low pH
C8-Fluorine
Chemical Compatibility & Mobile Phase Guidelines
- Compatible: aqueous buffers (acetate, phosphate, formate, Tris), organic modifiers (acetonitrile, methanol, ethanol, THF ≤10%), acidic additives (TFA ≤0.2%, formic acid ≤0.2%, phosphoric acid ≤0.1%), basic additives (ammonium hydroxide, triethylamine ≤0.1%)
- Not recommended: strong bases (>pH 10 Universal; >pH 8 Low pH/Fluorine), 10% THF, 80°C operation, chlorinated solvents (DCM, chloroform)
Ordering Information – Full SKU List
C8-Universal, 3μm (120Å)
- 1 mm ID (30-250 mm) -Cat# 13011291 to 13011299
- 0 mm ID (30-250 mm) -Cat# 13011300 to 13011307
- 0 mm ID (30-250 mm) -Cat# 13011308 to 13011317 (includes 3.9×150, 3.9×250)
- 6 mm ID (30-250 mm) -Cat# 13011318 to 13011326
C8-Universal, 5μm (120Å)
- 1 mm ID -Cat# 13011327 to 13011335
- 0 mm ID -Cat# 13011336 to 13011343
- 0 mm ID -Cat# 13011344 to 13011353 (includes 3.9×150, 3.9×250, 3.9×300, 4.0×300)
- 6 mm ID -Cat# 13011356 to 13011365 (includes 4.6×300)
- 0-7.8 mm ID (preparative) -Cat# 13011366 to 13011372
- 300Å pore, 5μm, 4.6×250 mm -Cat# 13011373
C8-Universal, 10μm (120Å)
- 9×300, 4.0×300, 4.6×150/200/250/300 -Cat# 13011374 to 13011379
C8-Low pH (3μm & 5μm, 120Å)
- Full range of dimensions (2.1, 3.0, 4.0, 4.6 mm ID; 30-300 mm lengths) -Cat# 13011380 to 13011462
- 300Å, 5μm, 4.6×250 mm -Cat# 13011462
C8-Fluorine (3μm & 5μm, 120Å)
- Full range of dimensions (2.1, 3.0, 4.0, 4.6 mm ID; 30-300 mm lengths) -Cat# 13011463 to 13011537
Download PDF Datasheet
Complete dimension and length details for Low pH and Fluorine are the same coverage as Universal. For a full PDF catalog, email info@hawach.com.
FAQ
What is the difference between C8 and C18?
C8 has a shorter alkyl chain (8 carbons) than C18 (18 carbons), resulting in less hydrophobic retention. C8 is often faster for strongly retained compounds.
Which C8 phase should I start with for method development?
C8-Universal is the most versatile. If your method requires low pH (<2) or 100% aqueous, choose C8-Low pH. For halogenated compounds, try C8-Fluorine.
Can I use 100% acetonitrile or methanol?
Yes, but avoid abrupt changes from high aqueous to high organic – use gradient.
Are these columns compatible with LC/MS?
Yes. Low bleed, 3 μm and 2.1 mm ID are ideal for LC/MS.
Do you offer guard columns?
Yes. Standard guard cartridges and holders are available for all column IDs.
What is the maximum pressure for 3 μm columns?
~6000 psi (400 bar) – suitable for UHPLC and conventional HPLC.
Do you offer custom dimensions (non-standard lengths/IDs)?
Yes. Minimum order quantity applies – contact OEM team.
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