ISO 12771
Red Color Code
Welded Tip
Gamma Irradiated
DNase/RNase-Free
Endotoxin-Free
Availability: In Stock
25 mL Serological Pipette
Description
Common Problems with Bulk-Packed Serological Pipettes
- Opened bags expose unused pipettes to airborne contaminants, moisture, and particulates
- Difficult aseptic transfer into biosafety cabinets – risk of touching non-sterile surfaces
- Higher waste in low-volume workflows – you must open many pipettes at once
- Increased risk of cross-contamination in GMP or cell therapy environments
Hawach individually wrapped sterile serological pipettes solve these issues – open one at a time, keep the rest sealed, and transfer aseptically into laminar flow hoods, significantly reducing contamination risk. The 25 mL size is the high-capacity workhorse for large-volume cell culture tasks such as feeding 175 cm² flasks or larger vessels, preparing bulk media, and transferring large sample volumes in QC and production workflows.
Product Overview
The 25 mL serological pipette – also referred to as a 25 mL cell culture pipette or sterile 25 mL serological pipette – is a precision disposable pipette designed for aseptic liquid handling where volumes of 15–25 mL are routinely needed. Each pipette is individually wrapped in a sterile peel-pouch, allowing you to open only what you need – ideal for GMP-controlled workflows and busy biosafety cabinets.
Hawach’s 25 mL sterile disposable pipette (Cat# 21111005) meets ISO 12771, features a welded tip for added strength and consistent internal diameter, and is color-coded red for easy size identification (standard for 25 mL). It is gamma irradiated (SAL 10⁻⁶), certified non-pyrogenic, DNase/RNase-free, and endotoxin-free. The large inner diameter (Φ14.4 mm) provides very fast liquid uptake, and the extended length (341.8 mm) ensures excellent media bottle access – you can reach the bottom of 1 L or 2 L bottles without tipping, significantly reducing spill risk during large-volume transfers.
Key advantage: Individual sterile packaging minimizes contamination exposure and waste – a critical upgrade from bulk-packed alternatives. The 25 mL format is the go-to pipette for routine media exchange in large culture vessels (175 cm² flasks, 150 mm dishes), bulk reagent preparation, and high-throughput liquid handling where 25 mL volumes are standard.
Why Labs Choose Individually Wrapped 25 mL Serological Pipettes
- Reduced contamination risk – Each pipette sealed until use. No exposure of unused pipettes to ambient air or bench surfaces.
- Better BSC workflow – Peel-open pouch enables aseptic transfer into laminar flow hoods without touching the pipette shaft.
- Lower waste – Open exactly the number needed. No leftover contaminated pipettes from opened bulk bags.
- GMP & QC ready – Individual packaging simplifies traceability and controlled-environment handling; lot numbers on every carton.
- Optimized 25 mL capacity for large vessels – Ideal for feeding 175 cm² flasks (20–25 mL media), 225 cm² flasks, 150 mm dishes, preparing bulk buffers, and transferring larger sample volumes in QC workflows.
- Excellent media bottle access – The 341.8 mm length reaches deep into 1 L or 2 L media bottles without tipping, making large-volume sterile liquid transfer safer and faster.
- Welded tip for extra strength – The tip is welded (not just stretched) to ensure consistent orifice size and prevent tip breakage during high-force aspirations or viscous liquids.
For cell therapy, vaccine production, and clinical diagnostics, individually wrapped serological pipettes are the standard over bulk-packed. The 25 mL cell culture pipette is especially popular for routine passage of cells in large vessels, bulk media preparation, and any workflow requiring 25 mL transfers.
Universal Pipettor Compatibility
Compatible with all major serological pipettors and electronic controllers that accept 25 mL pipettes (some controllers may require a larger adapter; please check your pipettor specifications). Hawach 25 mL pipettes have a standard top diameter that fits most 1-25 mL pipettors.
| Brand | Compatible? |
| Eppendorf | Yes (with 25 mL compatible models) |
| Thermo Scientific | Yes |
| Sartorius | Yes |
| Brand | Yes |
| Gilson | Yes |
| Rainin | Yes |
| Other standard 1-25 mL controllers | Yes (verify compatibility) |
Fits standard top diameter for 25 mL serological pipettes. The longer length (341.8 mm) is designed to reach the bottom of deep vessels while maintaining compatibility with standard pipettors.
Comparison: Individually Wrapped vs. Bulk-Packed
| Feature | Individually Wrapped (Hawach) | Bulk–Packed |
| Sterility after first opening | High – each pipette sealed | Medium – remaining pipettes exposed |
| Aseptic transfer into BSC | Easy – open pouch inside hood | Difficult – must handle carefully |
| Cross-contamination risk | Low | Higher |
| Suitable for GMP / regulated labs | Yes | Limited |
| Waste from unused pipettes | None | Some |
| Cost per pipette | Moderate (value for sterility) | Lower (but higher indirect costs) |
Conclusion: For aseptic cell culture and regulated workflows, individually wrapped pipettes provide superior sterility assurance.
Key Features
- Welded tip – Stronger than stretched tips; consistent orifice size reduces variability and prevents tip breakage during aspiration of viscous liquids or when working with large volumes.
- ISO 12771 color code – Red ring and graduation marks prevent size mix-
- Certified purity – Non-pyrogenic, DNase-free, RNase-free, endotoxin-free (≤0.25 EU/mL).
- High clarity polystyrene – Easy meniscus reading.
- Negative graduations – Allows blow-out delivery.
- Gamma irradiated (SAL 10⁻⁶) – Ready to use, no autoclaving.
- Lot traceability – COA available per lot.
- Extended length (341.8 mm) – Superior media bottle access; reduces spill risk.
- Largest inner diameter (Φ14.4 mm) – Fastest liquid uptake among standard serological pipettes, ideal for high-volume transfers.
Specifications
| Parameter | Detail |
| Cat# | 21111005 |
| Volume | 25 mL |
| Graduation | 0.5 mL (typical) |
| Color code | Red (ISO 12771 for 25 mL) |
| Tip design | Welded tip |
| Dimensions | Φ14.4 mm × 341.8 mm |
| Material | Polystyrene (PS) |
| Sterility | Gamma irradiated, SAL 10⁻⁶ |
| Purity | Non-pyrogenic, DNase/RNase-free, endotoxin-free (≤0.25 EU/mL) |
| Packaging | Individually wrapped peel-pouch; 25 pouches/bag; 8 bags/carton |
| Quantity per carton | 200 pipettes |
| Compliance | ISO 9001 certified |
Applications – Focus on Large Culture Vessels & Bulk Transfers
| Area | Typical Use |
| Cell culture (large flasks/dishes) | Feeding 175 cm² flasks (20–25 mL media), 225 cm² flasks, 150 mm dishes, adding 25 mL buffers or trypsin to roller bottles |
| Bulk media & buffer prep | Transferring 25 mL aliquots, preparing dilution series, reagent addition to 25 mL tubes or bottles |
| Molecular biology | Large-scale nucleic acid precipitation, enzyme preps, buffer preparation |
| Clinical diagnostics | Serum/plasma transfers for 25 mL tubes, large-volume QC samples |
| Pharmaceutical QC | Reagent addition for 25 mL test volumes, dissolution sampling, bulk formulation mixing |
| Microbiology | Inoculation of 25 mL broths, culture dilutions, media preparation |
| General lab | Large-volume serial dilutions, reagent prep, media aliquoting |
Pipette Size Selection Guide
| Volume | Best Use | Typical Flask / Vessel |
| 1 mL | Small aliquots, enzyme addition | 96-well plate, small tubes |
| 2 mL | Routine small media changes | 25 cm² flask, 6-well plate |
| 5 mL | Standard media change | 25 cm² flask, 6-well plate, 60 mm dishes |
| 10 mL | Larger transfers, feeding 75 cm² flasks | 75 cm² flask, 100 mm dishes, 500 mL bottles |
| 25 mL | High-volume transfers, feeding 175 cm² / 225 cm² flasks, bulk media prep | 175 cm², 225 cm² flasks, 150 mm dishes, 1 L–2 L bottles |
Quality & Supply Stability
- ISO 9001 certified manufacturing.
- Batch-to-batch consistency – each lot tested for volume accuracy, sterility, endotoxin.
- Large-scale production capacity – supports long-term OEM, distributor, and bulk procurement programs.
- Full traceability – lot numbers printed on cartons and inner bags; COA, sterility certificates, and endotoxin reports available per lot.
- Stable raw material sourcing – ensures uninterrupted supply for high-volume laboratories.
Hawach maintains consistent lot validation and traceable QC documentation, meeting the requirements of GMP, ISO, and regulatory audits.
Packaging & Ordering
| Specification | Detail |
| Primary | 1 pipette per sterile peel-pouch (paper-plastic) |
| Secondary | 25 pouches per inner bag |
| Tertiary | 8 bags per carton |
| Total per carton | 200 pipettes |
| Storage | Dry, 15–30 °C, away from sunlight |
Ordering: info@hawach.com. Free samples available for qualified labs.
Frequently Asked Questions
Do you provide lot-specific COA and sterility certificates?
Yes. For every production lot, we provide Certificate of Analysis (COA), sterility certificate, and endotoxin test report upon request.
Are the pipettes manufactured in cleanroom conditions?
Yes. Hawach serological pipettes are manufactured and packaged in ISO-classified cleanroom environments to ensure sterility and particulate control.
Can the packaging configuration be customized for distributors?
Yes. We offer OEM services including custom pouch printing (logo, part number, instructions), private label cartons, and custom bag counts. Minimum order quantity applies.
What is the graduation interval on the 25 mL pipette?
Typically 0.5 mL increments from 0 to 25 mL, plus negative graduations for blow-out delivery. Exact interval available on COA.
Is it compatible with all electronic pipettors?
Most 1-25 mL serological pipettors from major brands (Eppendorf, Thermo, Sartorius, Brand, Gilson, Rainin) are compatible. However, some older or mini models may not accept the larger diameter of 25 mL pipettes. Please verify your pipettor’s specifications or contact us for a compatibility check.
What is the advantage of a welded tip over a stretched tip?
A welded tip is stronger and has a more consistent inner diameter than a stretched tip. It is less likely to break during aspiration of viscous liquids or when repeatedly inserting the pipette into bottles. The welded design also ensures more uniform flow characteristics across pipettes, improving volume accuracy.
Why is the 25 mL size ideal for large cell culture vessels?
175 cm² flasks typically require 20–25 mL of media for routine feeding, and larger vessels (225 cm², 150 mm dishes) also use 20–25 mL. The 25 mL pipette allows single-pipette feeding without needing to combine multiple smaller pipettes, reducing handling and contamination risk. Its extended length (341.8 mm) also provides easy access to the bottom of tall media bottles.
Can I get a long-term supply agreement with fixed pricing?
Yes. Hawach offers annual contracts, volume discounts, and scheduled deliveries for distributors and large laboratories.
Request Samples or OEM Quotation
Hawach supplies sterile individually wrapped serological pipettes for:
- Cell culture laboratories (academic, biotech, vaccine production)
- Molecular biology facilities (PCR, qPCR, NGS)
- Clinical diagnostic laboratories
- Pharmaceutical QC environments
- OEM / private label distributors
Available services:
- OEM pouch printing (logo, part number, instructions)
- Custom carton labeling and barcoding
- Distributor bulk pricing (volume discounts)
- COA, sterility, and endotoxin documentation per lot
- Long-term supply agreements (fixed pricing, scheduled deliveries)
Contact Hawach for quotation, validation documents, or free evaluation samples.
Email: sales@hawach.com
Please include: laboratory name, application, estimated monthly usage (or carton quantity), and any specific packaging requirements.
We will respond with a targeted quote within 24 hours.
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