250-1000 mL, Virgin Polypropylene, for Large-Volume High-Speed Workflows
Compatible with Beckman, Thermo, Sorvall rotors.
Availability: In Stock
High Speed Centrifuge Bottles
Description
| Field | Value |
| Product classification | laboratory high-speed centrifuge bottle (250-1000 mL) |
| Material & design | USP Class VI virgin polypropylene, round body with reinforced base |
| Rated performance (size-dependent) | 250 mL: 27,000 x g / 500 mL: 13,500 x g / 1000 mL: 7,000 x g (validated with ≥80% fill) |
| Sterility | Non-sterile standard; autoclavable 121°C × 20 min |
| Purity assurance | DNase-free, RNase-free, endotoxin-free |
| Rotor compatibility | Beckman Avanti / Thermo Sorvall / Eppendorf large-volume high-speed rotors |
| Application type | Large-volume cell supernatant, protein precipitation, bacterial culture harvesting, bioprocessing bulk clarification |
| Limitation | Not for ultracentrifugation (>100,000 x g); requires ≥80% fill for max RCF |
Decision Table – Why Upgrade to Hawach High-Speed Bottles
| Problem | Solution (Hawach Design) | Outcome | Impact |
| Large-volume bottle deformation at high g-force | Reinforced wall thickness + radial rib design on base; validated up to 27,000x g (250 mL), 13,500x g (500 mL), 7,000x g (1000 mL) | Maintains round profile under rated RCF; no buckling | High (prevents rotor imbalance & sample loss) |
| Leakage from threaded cap under centrifugal force | Deep thread + silicone O-ring seal (optional upgrade available) | Zero visible leakage during high-speed runs | High (protects rotor and adjacent samples) |
| Sample residue on inner wall after decanting | Low-retention inner surface (smooth, minimal binding) | Higher recovery of proteins, antibodies, or cell-free supernatants | High (reduces waste of valuable biological fluids) |
| Poor batch consistency across large volume orders | ISO 9001:2024 + lot traceability + ≤1% dimensional tolerance | Stable fit in rotors and reproducible pelleting | Medium (essential for GMP/QC labs) |
| Incompatible with standard high-speed rotors | Dimensions matched to Beckman, Sorvall, Thermo large-bucket systems | Direct drop-in or simple adapter compatibility | Medium (reduces need for new rotors) |
Product Specification Overview
All bottles are supplied with leak-resistant screw caps (polypropylene with silicone liner). Sterile options available upon request (E-beam).
| SKU | Volume | Max RCF | Outer Diameter (mm) | Height (mm) | Packaging | Carton Qty |
| 11361001 | 250 mL | 27,000 x g | 61.8 | 127.7 | 6 pcs/bag | 10 bags – 60 pcs |
| 11361002 | 500 mL | 13,500 x g | 69.5 | 170 | 6 pcs/bag | 5 bags – 30 pcs |
| 11361003 | 500 mL | 13,500 x g | 74 | 170 | 6 pcs/bag | 5 bags – 30 pcs |
| 11361004 | 1000 mL | 7,000 x g | 98 | 179 | 6 pcs/bag | 2 bags – 12 pcs |
| 11361005 | 1000 mL | 7,000 x g | 97.5 | 185 | 6 pcs/bag | 2 bags – 12 pcs |
Note: 500 mL and 1000 mL variants are offered with different diameter/height to suit specific rotor buckets. Check compatibility table before ordering.
Rotor Compatibility Guidance
The following compatibility is based on common large-volume high-speed rotors. Free rotor verification available.
| Rotor / Centrifuge Model | 250 mL (Ø61.8mm) | 500 mL (Ø69.5mm) | 500 mL (Ø74mm) | 1000 mL (Ø98/97.5mm) |
| Beckman Avanti J-LA / J-10 series | Direct (with adapter) | Direct or adapter | Check clearance | Direct (specific buckets) |
| Thermo Sorvall SLA-3000 / RC-12 | Direct | Direct | Direct (wide buckets) | Direct (large buckets) |
| Eppendorf FA-45-6-30 (high-speed) | Adapter required | Not standard | No | No |
| Hettich / VWR general purpose | Yes | Yes | Yes | Check rotor manual |
Engineered for Large-Volume High-Speed Performance
- Thick-wall design with radial reinforcement– Prevents barrel distortion at elevated g- Validated to 27,000x g for 250 mL bottles, 13,500x g for 500 mL, and 7,000x g for 1000 mL.
- Leak-resistant screw cap– Deep thread engagement and optional silicone liner maintain seal up to rated RCF even with volatile or detergent-containing solutions.
- Smooth low-retention interior– Minimizes protein and cell debris adhesion. Improves recovery of clarified supernatants and reduces washing steps.
- Chemical & thermal resistance– USP Class VI PP withstands common biological buffers, mild acids/bases, alcohols, and repeated autoclaving (121°C, 20 min, ≤5 cycles).
- Precision dimensional control– Lot-to-lot diameter and height tolerance ≤1% ensures consistent fit in rotor buckets and adapters.
Operational Guidelines
Critical for maximum RCF: Fill volume must be ≥80% of nominal capacity (e.g., ≥200 mL for 250 mL bottle). Lower fill volumes increase risk of sidewall collapse or cap leakage under high g-force.
- Recommended fill: 80-100% for high-speed runs. Leave small air gap for thermal expansion when autoclaving.
- Autoclaving: 121°C, 20 minutes, up to 5 cycles. Inspect for cracking or deformation after repeated use.
- Chemical compatibility: PBS, Tris, HEPES, sucrose, CsCl (<1.6 g/mL), ethanol (≤70%), isopropanol, mild detergents.
Avoid: strong acids/bases (>1N), chlorinated solvents, phenol, chloroform, acetone. - Centrifuge balancing: Always use opposite bottles with equal fill weight. For swinging-bucket rotors, ensure caps are fully tightened but not over-
Performance Benchmark
Test protocol reference: ISO-like centrifugation condition (fixed-angle or swinging-bucket rotor, rated RCF per size, ≥80% fill, 25°C, 0.9% saline or PBS buffer) – internal validation for lab-grade benchmark definition.
| Metric | Generic Economy PP Bottles | Hawach High-Speed PP Bottles |
| Wall deformation at rated RCF | Noticeable bulging or oval shape (especially at 20,000x g for 250 mL) | No visible distortion up to 27,000x g (250 mL) / 13,500x g (500 mL) / 7,000x g (1000 mL) |
| Leakage rate (filled, capped, spun) | ~3-5% under max load | <1% (batch validated) |
| Protein recovery (BSA 1 mg/mL) | 82-86% | ≥92% (low-retention surface) |
| Autoclave cycles before degradation | 2-3 cycles | ≥5 cycles (reduced mechanical loss) |
| Dimensional consistency (OD) | ±2-3% | ≤1% (lot traceable) |
Application Intelligence
Typical large-volume high-speed workflows (matching bottle RCF capabilities):
- Harvesting bacterial or yeast cell pellets from 1-5 L cultures -250 mL / 500 mL bottles at 10,000-20,000x g
- Clarification of cell culture supernatants prior to protein purification -500-1000 mL bottles up to 13,500x g / 7,000x g
- Large-volume precipitation of nucleic acids or PEG-protein complexes -250-500 mL bottles up to 27,000x g or 13,500x g
- Bulk separation of microalgae or fine particles -250-1000 mL bottles
- Removal of cell debris from bioreactor harvests -1000 mL bottles at 7,000x g
- Industrial QC sample preparation (food, environmental, pharmaceutical) -any size
Application-Based Selection Guide
| Application | Recommended Size | Typical RCF Range | Key Bottle Feature |
| High-speed bacterial pellet (dense cultures) | 250 mL | 20,000-27,000 x g | Maximum mechanical strength (27,000x g rated) |
| Mammalian cell supernatant clarification | 500 mL | 8,000-13,500 x g | Low-retention surface |
| Protein precipitation (ammonium sulfate, PEG) | 250-500 mL | 12,000-15,000 x g | Chemical resistance, high RCF |
| Large-volume debris removal (bioprocessing) | 1000 mL | 5,000-7,000 x g | Autoclavable, wide neck, 7,000x g validated |
Procurement & Packaging
All bottles supplied with standard screw caps (PP). Sterile (E-beam) and bulk non-sterile options available upon request.
| SKU | Volume | Packaging Format | Bottles per Carton |
| 11361001 | 250 mL | 6/bag * 4 bags | 24 |
| 11361002 | 500 mL (Ø69.5) | 6/bag * 2 bags | 12 |
| 11361003 | 500 mL (Ø74) | 6/bag * 2 bags | 12 |
| 11361004 | 1000 mL (Ø98) | 6/bag * 3 bags | 18 |
| 11361005 | 1000 mL (Ø97.5) | 6/bag * 3 bags | 18 |
OEM & Bulk Supply Options
| Service | MOQ |
| Logo printing on bottle body | 20,000 pcs per size |
| Private label carton & bag | 500 cartons |
| Custom labelling (barcode, lot#) | 10,000 pcs |
| Annual fixed-price contract | ≥10,000 pcs/year (mix sizes) |
Documentation available: COA, DNase/RNase-free certificate, endotoxin report, sterility validation (if sterile ordered), ISO 9001 compliance.
Frequently Asked Questions
Can 500 mL bottles be used at 27,000 x g?
No. The 500 mL bottles are rated for 13,500 ×g maximum. Use the 250 mL size for applications requiring higher RCF.
Can these bottles be used in swinging-bucket rotors?
Yes, most are designed for swinging-bucket or fixed-angle large-volume rotors. Always check bucket internal diameter vs bottle outer diameter. Contact us for rotor-specific advice.
How many times can I autoclave them?
Recommended ≤5 cycles at 121°C for 20 minutes. After each cycle, inspect for haze, cracks, or loss of rigidity. Discard if damaged.
Do you offer sample bottles for testing?
Yes. Free samples (up to 2 bottles per size) available for qualified labs. Request via the form below.
What is the difference between 11361002 and 11361003 (both 500 mL)?
Different outer diameters (69.5 mm vs 74 mm) to fit specific rotor families. 11361002 fits tighter buckets (e.g., Sorvall), 11361003 fits wider buckets (e.g., Beckman). Consult rotor manual or send your rotor model.
Request Samples & Bulk Quotation
Free evaluation samples (up to 2 bottles per SKU)
Rotor compatibility verification – send your centrifuge model & rotor type
Bulk pricing, OEM customization, and long-term supply agreements
Response within 24 hours
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