40 um Cell Strainer

High-Precision Filtration for Single-Cell Suspension & Analytical Applications

The 40 µm cell strainer is designed for fine filtration of biological samples requiring highly uniform single-cell populations.

It is widely used in advanced cell analysis workflows where cell purity, consistency, and low background interference are critical, such as flow cytometry and single-cell sequencing.

Compared with larger pore sizes, the 40 µm strainer provides a higher level of filtration precision, making it suitable for downstream applications with strict analytical requirements.

What Is a 40 µm Cell Strainer?

A 40 µm cell strainer is a sterile laboratory filtration device with a uniform nylon mesh structure designed to separate single cells from tissue aggregates and debris.

Filtration Mechanism

During sample processing:

• Cell suspension is passed through a precision 40 µm nylon mesh
• Large aggregates, undigested tissue, and debris are retained on the surface
• Single cells pass through smoothly with minimal mechanical stress
• Controlled flow reduces shear-induced cell damage

This mechanism ensures high-purity single-cell output, which is essential for analytical accuracy.

Key Applications

• Flow cytometry (FACS) sample preparation
• Single-cell RNA sequencing (scRNA-seq) workflows
• Immune cell subset analysis
• Circulating cell and blood sample filtration
• High-precision cell sorting preparation
• Removal of aggregates before downstream molecular analysis

Performance Advantages

• High filtration precision for single-cell enrichment
• Improved accuracy in flow cytometry and sequencing data
• Effective removal of cell clumps and debris
• Reduces clogging in analytical instruments
• Stable pore structure ensures reproducibility
• Low cell activation and minimal mechanical stress

40 µm vs Other Cell Strainer Sizes

• Compared with 70 µm Cell Strainer → higher filtration precision, lower cell aggregation
• Compared with 100 µm Cell Strainer → significantly higher purity but lower total yield

Best use case:
The 40 µm cell strainer is optimized for high-resolution analytical workflows requiring maximum single-cell purity.

Workflow Position in Cell Preparation

In multi-step cell processing workflows:

• First step (coarse filtration): 100 µm Cell Strainer
• Intermediate step: 70 µm Cell Strainer
• Final refinement step: 40 µm Cell Strainer

The 40 µm strainer is typically used as the final purification stage before analysis.

Product Features

• Uniform high-density nylon mesh for precise pore control
• Medical-grade polypropylene housing for biocompatibility
• Purple color-coded design for fast identification
• Compatible with standard 50 mL centrifuge tubes
• Sterile, DNase/RNase-free, and non-pyrogenic
• Individually packaged for contamination-free handling

Product Specifications

• Product Name: 40 µm Cell Strainer
• Mesh Pore Size: 40 µm
• Mesh Material: Medical-grade nylon
• Housing Material: Polypropylene (PP)
• Color Code: Purple
• Compatibility: Standard 50 mL centrifuge tubes
• Sterility: Sterile, DNase/RNase-free, non-pyrogenic
• Packaging: Individual sterile packaging

Why Choose Hawach Cell Strainers?

• High-precision mesh manufacturing for consistent pore size
• Low protein adsorption for improved cell recovery quality
• Strict batch-to-batch quality control for experimental stability
• Suitable for advanced cell biology and molecular research
• Stable bulk supply with OEM and private labeling support

FAQ

Why use a 40 µm cell strainer instead of 70 µm?

Because it provides finer filtration, enabling higher single-cell purity required for sensitive analytical applications such as flow cytometry and single-cell sequencing.

What samples are suitable for 40 µm cell strainers?

It is commonly used for blood samples, immune cells, and pre-processed tissue suspensions requiring high-purity single-cell isolation.

Is the 40 µm cell strainer sterile?

Yes. Hawach cell strainers are pre-sterile, DNase/RNase-free, and non-pyrogenic for direct laboratory use.

Are cell strainers reusable?

No, they are designed for single use to prevent cross-contamination.

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