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HPLC Column

Hawach Scientific is a famous HPLC Column supplier in China, we specially produce and supply prepacked and empty HPLC Column to the global market and stationary phase with the full range of materials available such as C18 Universal, C18 low PH, C18 Aqueous, C8 Universal, C8 low PH, C8 Aqueous, C8-Fluorine, Phenyl, NH2, CN, Diol, SiO2, Polar-RP, etc. Our HPLC columns with high column efficiency and excellent shape peaks and low pressure for a longer lifetime, especially the C18 Universal column which is equivalent to brands like Waters, Agilent, and GL Science.

Hawach HPLC column can provide a wide range of applications and the highest separation performance. In addition, it has unparalleled reliability, reproducibility, and stability.

HPLC columns

Normal Phase HPLC Column
ItemPore sizeSurface
Area(m2/g)
Carbon loading pH RangeEnd-cappedPrice
SiO2 HPLC Column120Å320N/A1.5-10No
NH2 HPLC Column120Å320N/A1.5-10No
CN HPLC Column120Å3206%1.5-10No
Diol HPLC Column120Å3202.50%1.5-10No
AmiNo Acid HPLC Column120Å32017%1.5-10Yes
Reverse Phase HPLC Column
ItemPore SizeSurface
Area(m2/g)
Carbon loadingpH RangeEnd-cappedPrice
C18-Aqueous HPLC Column120Å32012%1.5-10Yes
C18-Universal HPLC Column120Å32017%1.5-10Yes
300Å908%
C18-Low PH HPLC Column120Å16010%2.0-8.0No
C18-Alkaline HPLC Column120Å32017%1.5-10No
C8-Universal HPLC Column120Å32012%1.5-10Yes
300Å90
C8-Low PH HPLC Column120Å3205.50%1.0-8.0No
C8-Fluorine HPLC Column120Å32012%1.5-10Yes
C1 HPLC Column120Å3204%1.5-10Yes
C4 HPLC Column120Å3208%1.5-10Yes
300Å903%
CN HPLC Column120Å3206%1.5-10No
Phenyl HPLC Column120Å32012%1.5-10Yes
300Å906%
Phenyl-Ether HPLC Column120Å32012%1.5-10Yes
Polar RP HPLC Column120Å32018%1.5-10Yes
300Å90
C30 HPLC Column120Å32017%1.5-10Yes
HILIC Amide HPLC Column120Å3207%2.0-8.0No

There are many types of column packing. As an HPLC column supplier, we mainly supply as following columns:

SiO2 column is based on spherical and fully porous silica gel with less than 10 ppm of metal impurities (Al, Fe, Ti, Zr, etc.). Because this type of column has a strong polarity, it is often used to separate highly polar analytes.
The C18-Aqueous column is the hydrophilic column that can increase your buffer salt ratio in the 100% water phase range and improve the peak shape to the greatest extent.
The C18-Universal column is a very universal column.
C18-Low PH column acid-resistant unsealed column. It is especially suitable for polar samples. If the baseline separation cannot be achieved on the ordinary C18 column, the peak time will be prolonged on the modified chromatographic column to achieve the separation purpose.
C18-Alkaline column mixed stationary phase, the filler can withstand strong alkali conditions of pH 12.5. Hydrophilic groups replace a large number of silanol groups, which can effectively prevent the interaction between alkaloids and silanol groups.

The retention capacity of the C8 column is weaker than that of the C18 column. When you need to obtain a shorter analysis time than the C18 column, this column is a suitable choice.

PFP HPLC column is their high resolution and retention of aromatic compounds, which can be difficult to separate using other stationary phases. PFP columns are also compatible with a wide range of solvents and can be used for both normal-phase and reversed-phase separations.

Selecting an HPLC Column

When choosing an HPLC column, consider the following factors:

  • Nature of the Sample: Polar or non-polar, ionic or neutral, large or small molecules.
  • Separation Mode: Reverse phase, normal phase, ion exchange, size exclusion, or chiral separation.
  • Column Dimensions: Length, inner diameter, and particle size based on the required resolution and available system pressure.
  • Mobile Phase Compatibility: The column should be compatible with the solvents in the mobile phase to ensure longevity and optimal performance.
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