Round Bottom, 10-100 mL, validated up to 50,000 x g
Suitable for popular centrifuges such as ThermoFisher, Sigma, Beckman, Eppendorf, Tomy, etc.
Availability: In Stock
High Speed Centrifuge Tubes
Description
Decision Table – Problems Solved with Hawach High-Speed Tubes
| Problem | Solution (Hawach Design) | Outcome | Impact |
| Tube deformation or cracking above 30,000 x g | Round bottom eliminates stress concentration; uniform wall thickness | Stable performance up to 50,000 x g | High (prevents rotor damage & sample loss) |
| Poor pellet recovery after high-speed run | Smooth inner surface + round geometry reduces resuspension during braking | Pellet recovery ≥90% (vs 80-85% generic) | High (critical for scarce samples) |
| Incompatibility with multiple rotor families | Precision dimensions matched to Beckman, Thermo, Eppendorf, Sorvall | Drop-in or adapter-based fit for most common high-speed rotors | Medium (reduces need for multiple tube types) |
| Uncertain chemical resistance after autoclaving | Virgin PP + validated 121°C cycles (≤5 recommended) | Maintains mechanical integrity after repeated sterilization | Medium (lowers consumable waste) |
| Risk of inner wall collapse under vacuum/ultra-high RCF | ≥80% fill volume warning + round bottom hydraulic support | Avoids implosion during high-speed runs | High (safety & equipment protection) |
Product Specification Overview
| SKU | Volume | Max RCF | Outer Diameter | Height | Rotor Compatibility |
| 11332001 | 10 mL | 50,000 x g | 16 mm | 81.4 mm | Beckman JA / Thermo SA / Eppendorf FA (direct) |
| 11332002 | 30 mL | 50,000 x g | 25.5 mm | 94.3 mm | Beckman JA / Thermo SA (direct or adapter) |
| 11332003 | 50 mL | 50,000 x g | 29 mm | 106.7 mm | Beckman JA / Thermo SA (adapter may be required) |
| 11332004 | 100 mL | 50,000 x g | 38 mm | 105.7 mm | Limited compatibility – check rotor manual |
Why Round Bottom Design Matters
Round bottom eliminates the stress concentration point at the cone-to-cylinder transition found in conical tubes. This improves structural integrity under high centrifugal force.
- Improved mechanical stability under high RCF
- Reduced risk of microfracture at high-speed operation
- Improved pellet integrity during deceleration
- Reduced resuspension during braking phase
Comparative advantage: Round-bottom design typically improves pellet recovery by 10–15% under high-speed conditions compared to conical tubes.
Rotor Compatibility Matrix
| Rotor Family | 10 mL | 30 mL | 50 mL | 100 mL |
| Beckman JA (JA-20 / JA-25 / JA-30) | Adapter | Direct or adapter | Adapter | Limited |
| Thermo SA Series | Adapter | Direct or adapter | Adapter | Check manual |
| Eppendorf FA Series | Direct | Limited | Not recommended | Not compatible |
| Sorvall SS-34 | Adapter | Adapter | Adapter | Not compatible |
Free rotor compatibility verification available upon request.
Operational Guidelines
⚠️ Critical requirement for 50,000 x g operation: Fill volume must be ≥80% of nominal capacity. Lower fill volumes increase risk of tube implosion or deformation.
- Recommended fill:≥80% of nominal volume
- Autoclaving:121°C, 20 min, ≤5 recommended cycles. Inspect for haze, cracking, or deformation after repeated use.
- Chemical compatibility:PBS, Tris, HEPES, sucrose gradients (≤60%), CsCl (≤1.6 g/mL), ethanol, isopropanol, mild detergents (Triton X-100, Tween-20).
Not compatible with: strong acids/bases (>1N), chlorinated solvents, phenol-chloroform, strong oxidizing agents.
Performance Benchmark
Test protocol reference: ISO-like centrifugation condition (fixed-angle rotor, 50,000 x g, ≥80% fill volume, 25°C, standard biological buffer) – internal validation protocol for lab-grade benchmark definition.
| Metric | Generic High–Speed Tubes | Hawach High–Speed Tubes |
| Leakage at 50,000 x g | Occasional | Minimized (validated design) |
| Wall deformation | Common above 30,000 x g | Stable under 50,000 x g (with ≥80% fill) |
| Pellet recovery | 80-85% | ≥90% under optimized protocol |
| Autoclave durability | 3-5 cycles | Up to 5 cycles (inspect after each) |
| Dimensional tolerance | ±2-3% | ≤1% batch consistency |
Application Intelligence
Typical high–speed applications:
- Subcellular fractionation (nuclei, mitochondria, microsomes)
- Viral particle concentration (AAV, lentivirus, bacteriophage)
- Exosome isolation workflows
- Nanoparticle washing (gold, silica, magnetic beads)
- Protein aggregate removal prior to LC-MS / HPLC analysis
- Cell debris clarification in bioprocessing
Application-Based Selection Guide
| Application | Recommended Size | Typical RCF Range |
| Virus concentration | 10-30 mL | 40,000-50,000 x g |
| Organelle isolation | 30-50 mL | 30,000-50,000 x g |
| Nanoparticle purification | 10-30 mL | 40,000-50,000 x g |
| Protein clarification | 50-100 mL | 30,000-50,000 x g |
Procurement Information
| SKU | Volume | Packaging | Carton Quantity |
| 11332001 | 10 mL | 10 pcs/bag * 10 bags | 100 pcs |
| 11332002 | 30 mL | 10 pcs/bag * 10 bags | 100 pcs |
| 11332003 | 50 mL | 10 pcs/bag * 10 bags | 100 pcs |
| 11332004 | 100 mL | 10 pcs/bag * 10 bags | 100 pcs |
OEM and Bulk Supply
| Service | MOQ |
| Logo printing on tube wall | 20,000 pcs per size |
| Private label carton | 500 cartons |
| Custom bag labeling | 10,000 pcs |
| Annual fixed-price contract | ≥20,000 pcs/year |
Documentation available upon request
- Certificate of Analysis (COA)
- DNase/RNase-free certification
- Endotoxin testing report
- Sterilization validation report
- ISO 9001 manufacturing compliance
Frequently Asked Questions
Why round bottom instead of conical tubes for high speed?
Round bottom eliminates stress concentration points, improving high-RCF stability and pellet integrity. It also reduces resuspension during braking, giving better recovery.
Can these tubes be used at 50,000 x g ?
Yes, all sizes are validated for 50,000 ×g provided that fill volume is ≥80% and you use appropriate rotors with full support. Check the compatibility matrix above.
Are they suitable for ultracentrifugation (>100,000 x g ?
No. For applications above 100,000 ×g, please use polycarbonate or polyallomer ultracentrifuge tubes (not polypropylene).
Can they be autoclaved repeatedly?
Yes, up to 5 cycles at 121°C for 20 minutes. After each cycle, inspect for haze, cracking, or deformation; discard if any damage is visible.
Are they compatible with Beckman JA and Thermo SA rotors?
Yes, with direct or adapter-based compatibility depending on the specific rotor and tube size. Free rotor compatibility verification available upon request.
What is the minimum order quantity?
One carton (100 pcs per SKU). Sample packs (up to 10 pcs per size) are available free of charge for evaluation.
Samples & Bulk Quotation
Free evaluation samples (up to 10 pcs per size)
Rotor compatibility verification – send your rotor model
Bulk pricing, OEM customization, and long-term supply agreements
Response within 24 hours
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