Available in particle sizes: 3μm, 5μm, 10μm
Pore sizes: 120Å, 300Å
Compatible with HPLC, UHPLC and LC-MS systems.
Availability: In Stock
C4 HPLC Column
Description
Hawach C4 HPLC Column is a reversed phase column packed with high-purity silica bonded with butyl (C4) functional groups. It provides moderate hydrophobic retention, excellent selectivity and reliable recovery for proteins, peptides and other large biomolecules.
Compared with C18 and C8 columns, the shorter alkyl chain of C4 results in weaker hydrophobic interaction, making it especially suitable for samples that require reduced retention and improved recovery of biological compounds.
Available in particle sizes: 3μm, 5μm, 10μm | Pore sizes: 120Å, 300Å | Compatible with HPLC, UHPLC and LC-MS systems.
Key Features
- Butyl bonded silica stationary phase
- Moderate hydrophobic retention -reduces excessive retention of large biomolecules
- Excellent protein and peptide recovery -ideal for biomolecule separation
- Multiple pore sizes -120Å and 300Å for different molecular weight ranges
- Flexible particle sizes -3μm, 5μm, 10μm
- High reproducibility -advanced packing technology ensures consistent performance
- Broad compatibility -works with conventional HPLC, UHPLC and LC-MS
C4 vs C8 vs C18 – Quick Comparison
| Column Type | Stationary Phase | Hydrophobicity | Retention | Best For |
| C4 | Butyl bonded silica | Low-Moderate | Lower | Proteins, peptides, biomolecules |
| C8 | Octyl bonded silica | Moderate | Medium | Peptides, pharmaceuticals, small molecules |
| C18 | Octadecyl bonded silica | High | Strong | General reversed phase HPLC |
Why Choose C4 HPLC Column?
Choose C4 when C18 provides excessive retention or insufficient recovery:
- Better recovery of proteins and peptides
- Reduced interaction with large biomolecules
- Improved peak shape for biological samples
- More efficient separation of complex samples
Applications
| Application Area | Typical Use |
| Protein Analysis | Protein separation, purity analysis, characterization, recombinant protein analysis |
| Peptide Analysis | Peptide mapping, purity testing, synthetic peptide analysis |
| LC-MS Analysis | High recovery, efficient desorption, stable performance |
| Biopharmaceutical | Protein drug analysis, peptide drug QC, biomolecule characterization |
| Method Development | Research and method optimization |
Pore Size Selection: 120Å vs 300Å
| Pore Size | Best For | Advantages |
| 120Å | Small peptides, peptide fragments, routine analytical HPLC | Higher efficiency, excellent peak shape, good separation performance |
| 300Å | Proteins, large peptides, biomolecules, recombinant proteins | Improved mass transfer, better recovery, reduced steric limitation |
Particle Size Selection: 3μm vs 5μm vs 10μm
| Particle Size | Best For | Features |
| 3μm | High-resolution analysis, UHPLC, advanced method development | Higher efficiency, better resolution, faster analysis |
| 5μm | Routine analytical HPLC, QC labs, general research | Balanced pressure and efficiency, excellent reproducibility |
| 10μm | Preparative chromatography, purification, large-scale separation | Higher loading capacity, lower pressure |
Technical Specifications
| Parameter | Specification |
| Stationary Phase | C4 (Butyl bonded silica) |
| Mode | Reversed Phase HPLC |
| Particle Sizes | 3μm, 5μm, 10μm |
| Pore Sizes | 120Å, 300Å |
| pH Range | pH 2-8 |
| Compatible Systems | HPLC, UHPLC, LC-MS |
| Applications | Proteins, peptides, biomolecules, pharmaceutical analysis |
Ordering Information – C4 HPLC Column (5μm, 120Å) – Selected SKUs
| Cat# | Description | Package |
| 13021173 | C4, 5μm, 120Å, 2.1×30mm | 1/pk |
| 13021175 | C4, 5μm, 120Å, 2.1×50mm | 1/pk |
| 13021177 | C4, 5μm, 120Å, 2.1×100mm | 1/pk |
| 13021179 | C4, 5μm, 120Å, 2.1×150mm | 1/pk |
| 13021181 | C4, 5μm, 120Å, 2.1×250mm | 1/pk |
| 13021187 | C4, 5μm, 120Å, 3.0×150mm | 1/pk |
| 13021195 | C4, 5μm, 120Å, 3.9×150mm | 1/pk |
| 13021198 | C4, 5μm, 120Å, 3.9×250mm | 1/pk |
| 13021208 | C4, 5μm, 120Å, 4.6×150mm | 1/pk |
| 13021210 | C4, 5μm, 120Å, 4.6×250mm | 1/pk |
| 13021211 | C4, 5μm, 120Å, 4.6×300mm | 1/pk |
Full SKU list available – including 3μm, 10μm, and 300Å pore sizes. Contact info@hawach.com for complete catalog.
Or download below.
Download PDF Datasheet
Storage and Maintenance
- Flush column after each analytical run
- Remove salts and buffer residues before storage
- Store in appropriate organic solvent with end plugs installed
- Avoid leaving biological samples inside column
- Follow recommended flow rates to prevent excessive pressure
FAQs
What is the difference between C4 and C18?
C4 has a shorter butyl chain (4 carbons) vs C18 (18 carbons), resulting in lower hydrophobicity and weaker retention. C4 is preferred for proteins and large biomolecules where C18 may cause excessive retention or poor recovery.
When should I choose C4 over C8?
Choose C4 for larger biomolecules (proteins, large peptides) that require minimal hydrophobic interaction and maximum recovery. C8 is better suited for peptides and small molecules.
Which pore size should I choose?
120Å for small peptides and routine analysis; 300Å for proteins and large biomolecules to ensure full pore accessibility.
Which particle size is best for LC-MS?
3μm with 2.1mm ID is ideal for LC-MS applications, providing high efficiency and low flow rates.
What pH range is recommended?
pH 2-8 for optimal performance and column lifetime.
Do you offer custom dimensions?
Yes – custom lengths and IDs available on request (MOQ applies). Contact OEM team for details.
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