Reversed-Phase
C8/SCX & C8/SAX Mixed-Mode
Availability: In Stock
C8 SPE Cartridge
Description
Product Overview
Hawach C8 SPE portfolio covers standard reversed-phase C8, mixed-mode C8/SCX cation-exchange, and C8/SAX anion-exchange phases. All sorbents are based on high-purity octyl-bonded silica, delivering single hydrophobic retention or dual-mode interaction for the selective extraction of neutral, acidic, and basic analytes from biological, food, and environmental matrices.
Sorbent Chemistry
Standard C8
Medium hydrophobic octyl phase with weaker retention than C18. Ideal for medium-polar compounds that require fast, complete elution and high sample loading capacity in routine sample preparation.
C8/SCX Mixed-Mode
Combines octyl reversed-phase retention with strong cation exchange functionality. Designed for simultaneous capture of hydrophobic compounds and positively charged basic analytes, including alkaloids, beta-blockers, and amine metabolites in biofluids.
C8/SAX Mixed-Mode
Integrates C8 hydrophobic interaction with strong anion exchange groups. Optimized for retaining medium-hydrophobic substances and negatively charged acidic analytes such as organic acids, phenolic compounds, and anionic pesticide residues.
Retention Mechanism
- C8 Reversed-Phase: Analyte retention via hydrophobic interaction; elution achieved by increasing organic solvent concentration.
- C8/SCX: Dual hydrophobic and cation-exchange retention. Selectivity adjustable by pH and ionic strength modification for basic compound cleanup.
- C8/SAX: Dual hydrophobic and anion-exchange retention for targeted extraction of acidic and polar anionic analytes.
Typical Laboratory Applications
- Plasma, serum, and urine cleanup for pharmaceutical metabolites and therapeutic drug monitoring
- Extraction of basic drugs, alkaloids, and positively charged small-molecule compounds
- Pretreatment of organic acids, phenolics, and acidic food additives
- Trace analysis of semi-volatile organic compounds in environmental water samples
- Pesticide residue screening in food and agricultural extracts
- Forensic toxicology and controlled substance extraction from biological matrices
C8 vs C18 SPE Technical Comparison
| Parameter | C8 SPE | C18 SPE |
| Hydrophobic Retention | Moderate | Strong |
| Elution Characteristic | Faster, complete elution | Tighter binding, slower elution |
| Best Analyte Match | Medium-polar compounds, weak hydrophobic targets | Strongly hydrophobic non-polar analytes |
| Matrix Cleanup | Effective for complex biofluid and food extracts | Suitable for general environmental reversed-phase workflows |
| Primary Usage | High-throughput bioanalysis, polar compound extraction | Universal trace organic enrichment |
Sorbent Selection Guide
- Medium-polar neutral analytes requiring fast elution -Standard C8
- Basic drugs, alkaloids, amine metabolites in biofluids -C8/SCX
- Organic acids, phenolics, anionic contaminants -C8/SAX
- Complex multi-analyte matrices -Combined mixed-mode phase configuration
Technical Specifications
| Base Silica | High-purity spherical silica |
| Particle Size | 40-60 μm |
| Pore Size | 120 Å |
| Carbon Load | 8% |
| pH Stability | 2.0 – 10.0 |
Standard SPE Workflow
- Condition with methanol or acetonitrile
- Equilibrate with aqueous buffer
- Load sample at consistent flow rate
- Wash to remove polar matrix interferences
- Elute target analytes with organic or buffered eluents
Ordering Specifications
| Cartridge Volume | Sorbent Mass | Available Phases |
| 1 mL | 100 mg | C8 / C8/SCX / C8/SAX |
| 3 mL | 200 mg / 500 mg | C8 / C8/SCX / C8/SAX |
| 6 mL | 500 mg / 1 g | C8 / C8/SCX / C8/SAX |
| 12 mL | 1 g / 2 g | C8 / C8/SCX / C8/SAX |
Lab & Distributor Benefits
- Consistent batch-to-batch performance for regulated and high-throughput laboratories
- Fully compatible with standard SPE manifolds, positive pressure systems, and automated SPE workstations
- Method-ready phases for common pharmaceutical, environmental, and food safety analytical protocols
- Custom OEM packaging, private labeling, and bulk volume pricing available
- Full lot traceability and COA documentation for GLP and laboratory audit compliance
- Stable stock and short lead times for long-term institutional and distributor supply agreements
Order Information
C8 Cartridges
| Cat# | Description |
| 15011073 | SPE Cartridge, C8, 40-75μm, 100Å, 100mg/1mL, 100pcs/pk |
| 15011074 | SPE Cartridge, C8, 40-75μm, 100Å, 200mg/3mL, 50pcs/pk |
| 15011075 | SPE Cartridge, C8, 40-75μm, 100Å, 500mg/3mL, 50pcs/pk |
| 15011076 | SPE Cartridge, C8, 40-75μm, 100Å, 500mg/6mL, 30pcs/pk |
| 15011077 | SPE Cartridge, C8, 40-75μm, 100Å, 1g/6mL, 30pcs/pk |
| 15011078 | SPE Cartridge, C8, 40-75μm, 100Å, 2g/12mL, 20pcs/pk |
C8/SCX Cartridges
| Cat# | Description |
| 15011112 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 30mg/1mL, 100pcs/pk |
| 15011113 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 100mg/1mL, 100pcs/pk |
| 15011114 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 200mg/3mL, 50pcs/pk |
| 15011115 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 500mg/3mL, 50pcs/pk |
| 15011116 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 200mg/6mL, 30pcs/pk |
| 15011117 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 500mg/6mL, 30pcs/pk |
| 15011118 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 1g/6mL, 30pcs/pk |
| 15011120 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 1g/12mL, 20pcs/pk |
| 15011121 | SPE Cartridge, C8/SCX, 40-75μm, 70Å, 2g/12mL, 20pcs/pk |
C8/SAX Cartridges
| Cat# | Description |
| 15011122 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 30mg/1mL, 100pcs/pk |
| 15011123 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 100mg/1mL, 100pcs/pk |
| 15011124 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 200mg/3mL, 50pcs/pk |
| 15011125 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 500mg/3mL, 50pcs/pk |
| 15011126 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 200mg/6mL, 30pcs/pk |
| 15011127 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 500mg/6mL, 30pcs/pk |
| 15011128 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 1g/6mL, 30pcs/pk |
| 15011129 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 1g/12mL, 20pcs/pk |
| 15011130 | SPE Cartridge, C8/SAX, 40-75μm, 70Å, 2g/12mL, 20pcs/pk |
Frequently Asked Questions
What is the difference between C8 and C18 SPE cartridges?
C8 provides moderate hydrophobic retention with faster elution, ideal for medium-polar analytes that are prone to incomplete recovery on C18. C18 SPE cartridges offers stronger retention for non-polar, highly hydrophobic compounds.
When should C8/SCX mixed-mode SPE be used?
C8/SCX is preferred for extracting basic drugs, alkaloids, and positively charged metabolites from plasma and urine, where reversed-phase-only sorbents deliver insufficient selectivity.
What analytes are suitable for C8/SAX?
C8/SAX is optimized for acidic compounds, including organic acids, phenolic substances, anionic pesticides, and polar water-soluble contaminants.
Are mixed-mode C8 phases more selective than standard reversed-phase?
Yes. Dual hydrophobic and ion-exchange interactions reduce matrix interference, improving purification efficiency for complex biological and food extracts.
Can C8 SPE cartridges be used for LC-MS sample preparation?
Yes. C8, C8/SCX, and C8/SAX phases are widely used for LC-MS/MS bioanalysis, trace pollutant screening, and food residue quantification.
The quality has a guarantee
Fast & safe shipping worldwide
Contact Hawach for a Quotation
Enter your email address and our sales staff will
contact you as soon as possible.
