ISO 12771
Purple Color Code
Gamma Irradiated
DNase/RNase-Free
Endotoxin-Free
Availability: In Stock
50 mL Serological Pipette
Description
Common Problems with Bulk-Packed Serological Pipettes
- Opened bags expose unused pipettes to airborne contaminants, moisture, and particulates
- Difficult aseptic transfer into biosafety cabinets -risk of touching non-sterile surfaces
- Higher waste in low-volume workflows -you must open many pipettes at once
- Increased risk of cross-contamination in GMP or cell therapy environments
Hawach individually wrapped sterile serological pipettes solve these issues – open one at a time, keep the rest sealed, and transfer aseptically into laminar flow hoods, significantly reducing contamination risk. The 50 mL size is the maximum-capacity workhorse for large-volume cell culture tasks such as feeding 225 cm² and 300 cm² flasks, roller bottles, bulk media preparation, and high-volume QC workflows.
Product Overview
The 50 mL serological pipette – also referred to as a 50 mL cell culture pipette or sterile 50 mL serological pipette – is a precision disposable pipette designed for aseptic liquid handling where volumes of 25-50 mL are routinely needed. Each pipette is individually wrapped in a sterile peel-pouch, allowing you to open only what you need – ideal for GMP-controlled workflows and busy biosafety cabinets.
Hawach’s 50 mL sterile disposable pipette (Cat# 21111006) meets ISO 12771, features a welded tip for added strength and consistent internal diameter, and is color-coded purple for easy size identification (standard for 50 mL). It is gamma irradiated (SAL 10⁻⁶), certified non-pyrogenic, DNase/RNase-free, and endotoxin-free. The largest inner diameter (Φ17.7 mm) provides very fast liquid uptake, and the extended length (360 mm) ensures excellent media bottle access – you can reach the bottom of 2 L or 3 L bottles without tipping, significantly reducing spill risk during large-volume transfers.
Key advantage: Individual sterile packaging minimizes contamination exposure and waste – a critical upgrade from bulk-packed alternatives. The 50 mL format is the go-to pipette for routine media exchange in very large culture vessels (225 cm², 300 cm² flasks, roller bottles), bulk reagent preparation, and high-throughput liquid handling where 50 mL volumes are standard.
Why Labs Choose Individually Wrapped 50 mL Serological Pipettes
- Reduced contamination risk– Each pipette sealed until use. No exposure of unused pipettes to ambient air or bench surfaces.
- Better BSC workflow– Peel-open pouch enables aseptic transfer into laminar flow hoods without touching the pipette shaft.
- Lower waste– Open exactly the number needed. No leftover contaminated pipettes from opened bulk bags.
- GMP & QC ready– Individual packaging simplifies traceability and controlled-environment handling; lot numbers on every carton.
- Optimized for very large vessels– Ideal for feeding 225 cm² flasks (40-50 mL media), 300 cm² flasks, roller bottles, preparing bulk buffers, and transferring large sample volumes in production QC workflows.
- Excellent media bottle access– The 360 mm length reaches deep into 2 L or 3 L media bottles without tipping, making very large-volume sterile liquid transfer safer and faster.
- Welded tip for extra strength– The tip is welded (not just stretched) to ensure consistent orifice size and prevent tip breakage during high-force aspirations or viscous liquids.
For cell therapy, vaccine production, and clinical diagnostics, individually wrapped serological pipettes are the standard over bulk-packed. The 50 mL cell culture pipette is essential for laboratories that routinely handle 50 mL transfers, such as large-scale bioreactor sampling, bulk media preparation, and high-volume QC workflows.
Universal Pipettor Compatibility
Compatible with serological pipettors that accept 50 mL pipettes. Note: Not all standard 1-10 mL pipettors can handle 50 mL pipettes due to the larger top diameter and longer length. Please verify your pipettor specifications.
- Eppendorf (with 50 mL compatible models)
- Thermo Scientific
- Sartorius
- Brand
- Gilson (check model)
- Rainin
- Other 1-50 mL universal controllers
Comparison: Individually Wrapped vs. Bulk-Packed
| Feature | Hawach Individually Wrapped | Common Bulk – Packed |
| Sterility after first opening | High – each pipette sealed | Medium – remaining pipettes exposed |
| Aseptic transfer into BSC | Easy – open pouch inside hood | Difficult – must handle carefully |
| Cross-contamination risk | Low | Higher |
| Suitable for GMP / regulated labs | Yes | Limited |
| Waste from unused pipettes | None | Some |
| Cost per pipette | Moderate (value for sterility) | Lower (but higher indirect costs) |
Conclusion: For aseptic cell culture and regulated workflows, individually wrapped pipettes provide superior sterility assurance.
Key Features
- Welded tip – Stronger than stretched tips; consistent orifice size reduces variability and prevents tip breakage.
- ISO 12771 color code – Purple ring and graduation marks prevent size mix-
- Certified purity – Non-pyrogenic, DNase/RNase-free, endotoxin-free (≤0.25 EU/mL).
- High clarity polystyrene – Easy meniscus reading.
- Negative graduations – Supports blow-out delivery.
- Gamma irradiated (SAL 10⁻⁶) – Ready to use, no autoclaving.
- Lot traceability – COA available per lot.
- Extended length (360 mm) – Superior deep-bottle access; reduces spill risk.
- Largest inner diameter (Φ17.7 mm) – Fastest liquid uptake among all serological pipettes.
Technical Specifications
| Parameter | Specification |
| Catalog Number | 21111006 |
| Volume | 50 mL |
| Graduation Interval | 1.0 mL (typical) |
| Color Code | Purple (ISO 12771) |
| Tip Design | Welded tip |
| Dimensions | Φ17.7 mm × 360 mm |
| Material | Polystyrene (PS) |
| Sterility | Gamma irradiated, SAL 10⁻⁶ |
| Purity | Non-pyrogenic, DNase/RNase-free, endotoxin-free (≤0.25 EU/mL) |
| Packaging | Individually wrapped peel-pouch; 25 pouches/bag; 4 bags/carton |
| Quantity per carton | 100 pipettes |
| Compliance | ISO 9001 certified |
Main Applications
- Cell culture (large vessels): Feeding 225 cm² flasks (40-50 mL), 300 cm² flasks, roller bottles – single-pipette feeding reduces handling and contamination risk.
- Bulk media & buffer preparation: Transferring 50 mL aliquots, large-volume dilution series, reagent addition to 50 mL tubes or bottles.
- Molecular biology: Large-scale nucleic acid precipitation, enzyme preps, buffer preparation.
- Clinical diagnostics: Serum/plasma transfers for 50 mL centrifuge tubes, large-volume QC samples.
- Pharmaceutical QC: Reagent addition for 50 mL test volumes, dissolution sampling, bulk formulation mixing.
- Microbiology: Inoculation of 50 mL broths, culture dilutions, media preparation for shake flasks.
- General laboratory: Very large-volume serial dilutions, reagent prep, bioreactor liquid distribution.
Serological Pipette Size Selection Guide
| Volume | Best Use | Typical Flask / Vessel |
| 1 mL | Small aliquots, enzyme addition | 96-well plate, small tubes |
| 2 mL | Routine small media changes | 25 cm² flask, 6-well plate |
| 5 mL | Standard media change | 25 cm² flask, 6-well plate, 60 mm dishes |
| 10 mL | Larger transfers, feeding 75 cm² flasks | 75 cm² flask, 100 mm dishes, 500 mL bottles |
| 25 mL | High-volume transfers, feeding 175 cm² flasks | 175 cm² flask, 150 mm dishes, 1-2 L bottles |
| 50 mL | Maximum-volume transfers, feeding 225-300 cm² flasks, roller bottles, bulk media prep | 225 cm², 300 cm² flasks, roller bottles, 2-3 L bottles |
Quality Assurance & Stable Supply
- ISO 9001 certified: manufacturing with strict batch-to-batch consistency.
- Large-scale production capacity– supports long-term OEM, distributor, and bulk procurement programs.
- Full traceability– lot numbers printed on cartons and inner bags; COA, sterility certificates, and endotoxin reports available per lot.
- Stable raw material sourcing– ensures uninterrupted supply for high-volume laboratories.
Packaging & Storage
- Primary: 1 pipette per sterile peel-pouch (paper-plastic).
- Secondary: 25 pouches per inner bag.
- Tertiary: 4 bags per carton – total 100 pipettes per carton.
- Storage: Dry, 15-30 °C, away from sunlight.
Frequently Asked Questions
Do you provide lot‑specific COA and sterility certificates?
Yes. For every production lot, we provide Certificate of Analysis (COA), sterility certificate, and endotoxin test report upon request.
Are the pipettes manufactured in cleanroom conditions?
Yes. Hawach serological pipettes are manufactured and packaged in ISO‑classified cleanroom environments to ensure sterility and particulate control.
Can the packaging configuration be customized for distributors?
Yes. We offer OEM services including custom pouch printing (logo, part number, instructions), private label cartons, and custom bag counts. Minimum order quantity applies.
What is the advantage of a welded tip over a stretched tip?
A welded tip is stronger and has a more consistent inner diameter than a stretched tip. It is less likely to break during aspiration of viscous liquids or when repeatedly inserting the pipette into bottles. The welded design also ensures more uniform flow characteristics across pipettes, improving volume accuracy – especially important for large 50 mL volumes.
Why is the 50 mL size ideal for the largest cell culture vessels?
225 cm² flasks typically require 40-50 mL of media, and 300 cm² flasks and roller bottles use 50 mL. The 50 mL pipette allows single‑pipette feeding without needing to combine multiple smaller pipettes, reducing handling and contamination risk. Its extended length (360 mm) also provides easy access to the bottom of tall 2-3 L media bottles.
Are these pipettes RNase/DNase‑free?
Yes. Each batch is certified free of DNase and RNase activity, non‑pyrogenic, and ≤0.25 EU/mL endotoxin.
Is OEM / private label available for the packaging?
Yes. We offer OEM pouch printing (logo, part number, barcode), custom carton labeling, and private label packaging. Minimum order quantity applies.
Request Samples or OEM Quotation
Hawach supplies sterile individually wrapped serological pipettes for:
- Cell culture laboratories (academic, biotech, vaccine production)
- Molecular biology facilities (PCR, qPCR, NGS)
- Clinical diagnostic laboratories
- Pharmaceutical QC environments
- OEM / private label distributors
Available services:
- OEM pouch printing (logo, part number, instructions)
- Custom carton labeling and barcoding
- Distributor bulk pricing (volume discounts)
- COA, sterility, and endotoxin documentation per lot
- Long-term supply agreements (fixed pricing, scheduled deliveries)
Contact Hawach for quotation, validation documents, or free evaluation samples.
Email: info @hawach.com
Please include: laboratory name, application, estimated monthly usage (or carton quantity), and any specific packaging requirements. We will respond with a targeted quote within 24 hours.
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