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Diol HPLC Column

Column: XChroma Diol HPLC Column, 5μm, 7.8×300mm
Wavelength: 276nm
Mobile Phase: Arginine solution(Preparing 1 mg/mL of L -arginine)/Ambient/Glacial acetic
Temperature: acid=65/20/15 30°C
Flow Rate: 0.5mL/min

Availability: In Stock

Description

Description of Diol HPLC Column

The diol column is a hydrophilic chromatographic column with diol groups bonded to a silica matrix. For highly polar compounds that have poor retention on reversed-phase columns, good retention can be obtained with eluents containing high concentrations of organic solvents. It has a good separation effect on polar compounds, and it is also used for the determination of amino acids.

Diol HPLC

Advantages Diol Column

  • 2,3-Dihydroxypropylsilane bonded silica gel stationary phase;
  • Diol HPLC column is commonly used as chromatographic columns for normal phase analysis, reverse mobile phase can also be used;
  • High surface coverage;
  • Ultra inertness and high durability;
  • Guaranteed reproducibility;
  • Excellent separation rate and pressure;
  • Reduce the influence of raw material silicone;
  • More stable than the traditional normal phase columns, such as Silica, and Amine;
  • Could be used in the reverse-phase analysis;
  • Similar polarity to the Amino column;
  • Good selectivity without excessive retention;
  • Improved peak shape versus bare silica.

Features

  • It can be used as a stationary phase of HILIC hydrophilic function chromatography;
  • Improved peak shape versus bare silica;
  • Stability and reproducibility are good in positive phase columns.
Bonded PhaseUSP No.Particle SizePore SizeSurface Area(m2)Carbon LoadingpH rangeEnd-capped
DiolL205 μm120Å3203%1.5-10No

Applications

  1. Suitable for most normal phase chromatography applications;
  2. The separation of organic acids and their polymers.

Hawach Scientific Co., Ltd Diol HPLC Column, the stationary phase is based on ultra-pure porous silica gel matrix, through the use of a unique fixed phase bonding technology, the use of 1, 2–dihydroxy propyl functional groups containing organosilane bonding. The Diol column is more stable than the traditional normal phase columns, such as NH2 HPLC columns, and SiO2 HPLC columns.

Diol-HPLC-Columns_2

Ordering Information

Item NoDescriptionPackage (pcs/pk)
13011799Diol, 3μm, 120Å, 2.1x30mm1
13011800Diol, 3μm, 120Å, 2.1x33mm1
13011801Diol, 3μm, 120Å, 2.1x50mm1
13011802Diol, 3μm, 120Å, 2.1x75mm1
13011803Diol, 3μm, 120Å, 2.1x100mm1
13011804Diol, 3μm, 120Å, 2.1x125mm1
13011805Diol, 3μm, 120Å, 2.1x150mm1
13011806Diol, 3μm, 120Å, 3.0x30mm1
13011807Diol, 3μm, 120Å, 3.0x50mm1
13011808Diol, 3μm, 120Å, 3.0x75mm1
13011809Diol, 3μm, 120Å, 3.0x100mm1
13011810Diol, 3μm, 120Å, 3.0x125mm1
13011811Diol, 3μm, 120Å, 3.0x150mm1
13011812Diol, 3μm, 120Å, 3.0x200mm1
13011813Diol, 3μm, 120Å, 3.0x250mm1
13011814Diol, 3μm, 120Å, 4.0x30mm1

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