3 μm / 5 μm / 10 μm
120Å
High Carbon Load
Baseline Resolution of 16 EPA Priority PAHs
Availability: In Stock
PAH HPLC Column – Optimized C18 Phase for EPA Method 610 PAH Analysis
Description
Product Overview
A PAH HPLC column is a reversed-phase C18 column engineered for high-resolution separation of polycyclic aromatic hydrocarbons (PAHs), a class of priority environmental pollutants regulated under EPA Method 610.
Unlike conventional C18 columns, PAH-dedicated phases are designed with enhanced bonding density and controlled surface chemistry to improve shape selectivity, enabling baseline separation of all 16 EPA priority PAHs using standard water/acetonitrile gradient conditions.
Hawach PAH HPLC columns are produced from ultra-high purity (>99.999%) fully porous spherical silica, providing high carbon loading, strong retention of planar aromatic compounds, and consistent column efficiency across production batches. Each batch is tested using EPA 610 PAH standard mixtures to ensure reproducible separation performance.
Available particle sizes include 3 μm (fast UHPLC), 5 μm (standard HPLC), and 10 μm (preparative applications). Multiple internal diameters are available, including 2.1 mm ID for LC/MS workflows and 4.6 mm ID for routine environmental analysis.
Performance Highlights
- EPA Method 610-oriented design for full resolution of 16 priority PAHs
- High carbon load (~22%) for enhanced π-π interaction with aromatic analytes
- Scalable particle sizes: 3 μm / 5 μm / 10 μm for UHPLC, HPLC, and preparative use
- 120Å pore structure optimized for low-molecular-weight aromatic hydrocarbons (128-278 Da)
- Broad dimensional coverage: 2.1 mm ID (LC/MS), 4.6 mm ID (analytical), larger IDs for preparative workflows
- Method compatibility with Waters PAH C18, Agilent ZORBAX Eclipse PAH, Restek Pinnacle II PAH, and Supelcosil LC-PAH platforms
Column Selection Guide – Particle Size & Application Fit
| Application | Recommended Part No. | Particle Size | ID × Length | Performance Rationale |
| 16 EPA PAHs (routine environmental QC) | 13021008 | 5 μm | 4.6×250 mm | Standard EPA configuration with full baseline resolution |
| Fast analysis / UHPLC workflows | 13021005 | 3 μm | 4.6×250 mm | Higher efficiency, shorter runtime |
| LC/MS PAH profiling | 13021001 / 13021002 | 3 μm | 2.1×100 / 150 mm | Narrow bore for sensitivity and solvent reduction |
| Method development screening | 13021003 | 3 μm | 4.6×150 mm | Balanced resolution and speed |
| Preparative purification | Contact sales | 5-10 μm | 6.0-10.0 mm ID | High loading capacity for isolation workflows |
Recommended standard configuration:
13021008 – 5 μm, 4.6×250 mm for EPA Method 610 routine analysis (in stock, 3-5 day delivery)
Particle Size Selection Guide
| Particle Size | Best Application | Typical Runtime | Pressure Limit |
| 3 μm | UHPLC / LC/MS high throughput | ~15-20 min | ~6000 psi |
| 5 μm | Standard EPA Method 610 HPLC | ~25-35 min | ~4000 psi |
| 10 μm | Preparative purification | Extended | ~2500 psi |
Core Technical Specifications
| Parameter | Value |
| Bonded phase | High-density C18 optimized for aromatic selectivity |
| Particle sizes | 3 μm / 5 μm / 10 μm |
| Pore size | 120Å |
| Surface area | ~320 m²/g |
| Carbon load | ~22% |
| pH range | 2.0-8.0 |
| End-capping | Double end-capped |
| Max pressure | ~6000 psi (3 μm), ~4000 psi (5 μm) |
| Temperature limit | ≤60°C |
| Mobile phase | Water / Acetonitrile gradient (EPA Method 610) |
| Detection | UV 254 nm, FLD, LC/MS compatible |
Ordering Information – SKU Portfolio
| Cat# | Description | Package(pcs/pk) |
| 13021001 | PAH, 3μm, 120Å, 2.1 x 100 mm | 1 |
| 13021002 | PAH, 3μm, 120Å, 2.1 x 150 mm | 1 |
| 1302100 3 | PAH, 3μm, 120Å, 4.6 x 150 mm | 1 |
| 13021004 | PAH, 3μm, 120Å, 4.6 x 200 mm | 1 |
| 13021005 | PAH, 3μm, 120Å, 4.6 x 250 mm | 1 |
| 13021006 | PAH, 5μm, 120Å, 4.6 x 150 mm | 1 |
| 13021007 | PAH, 5μm, 120Å, 4.6 x 200 mm | 1 |
| 13021008 | PAH, 5μm, 120Å, 4.6 x 250 mm | 1 |
| 13021009 | PAH, 10μm, 120Å, 4.6 x 250 mm | 1 |
Typical Applications
| Application Area | Sample Types | Analytical Purpose |
| Environmental monitoring | Water, soil, sediment, air particulates | EPA compliance testing of PAHs |
| Food safety | Smoked foods, oils, seafood | Carcinogenic PAH detection |
| Industrial hygiene | Workplace air, emissions | Exposure monitoring |
| Petrochemical analysis | Crude oil, fuels | Hydrocarbon profiling |
| Pharmaceutical QC | API impurities | Aromatic impurity profiling |
| Research | Toxicology, environmental fate | Mechanistic studies |
Retention Mechanism
PAH separation is driven primarily by π-π interactions between aromatic analytes and the hydrophobic C18 bonded surface.
Key contributing factors:
- High carbon density increases interaction probability with planar aromatic structures
- Controlled surface chemistry reduces silanol interference and improves peak symmetry
- 120Å pore structure ensures efficient mass transfer for small aromatic molecules
This results in improved selectivity for structural PAH isomers that are difficult to resolve on conventional C18 phases.
Standard EPA Method 610 PAHs
Naphthalene, Acenaphthylene, Acenaphthene, Fluorene, Phenanthrene, Anthracene, Fluoranthene, Pyrene, Benzo[a]anthracene, Chrysene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[a]pyrene, Dibenzo[a,h]anthracene, Benzo[g,h,i]perylene, Indeno[1,2,3-c,d]pyrene
Chromatographic Conditions (EPA 610)
| Parameter | Condition |
| Column | 13021008, 5 μm, 4.6×250 mm |
| Mobile phase A | Water |
| Mobile phase B | Acetonitrile |
| Gradient | 50% → 100% B (20-30 min) |
| Flow rate | 1.0 mL/min |
| Column temp | 30°C |
| Detection | UV 254 nm / FLD |
| Runtime | 25-35 min (5 μm) |
Method Compatibility
Compatible with:
- EPA Method 610
- EPA Method 8310
- EPA Method 550
- ISO 17993
- DIN 38407-F8
Equivalent Column Compatibility
Waters PAH C18
Agilent ZORBAX Eclipse PAH
Restek Pinnacle II PAH
Supelco LC-PAH
Phenomenex Luna PAH
All equivalent systems operate under identical gradient and mobile phase conditions.
Manufacturing & Quality System
- ISO 9001 certified production
- 999% ultra-pure silica substrate
- Controlled high-density C18 bonding process
- Batch testing with EPA 610 PAH standard mix
- Full traceability per production lot
- COA and chromatographic test report included
Why Laboratories Choose Hawach PAH Columns
- Cost-effective alternative to major PAH column brands
- Verified EPA Method 610 performance per batch
- Drop-in replacement with no method change required
- Stable global supply chain for analytical continuity
- Fast delivery for standard SKUs
- Full regulatory documentation support (COA + chromatograms)
Frequently Asked Questions
What EPA methods are compatible with this column?
This column is designed specifically for EPA Method 610 (priority pollutant PAHs in water) and also supports EPA Method 8310 (solid waste), EPA Method 550 (drinking water), ISO 17993, and DIN 38407-F8.
What is the difference between a PAH column and a standard C18 column?
PAH columns use higher carbon loading (~22% vs ~15-17%), proprietary bonding techniques for enhanced shape selectivity, and are lot-tested with PAH standards to baseline-resolve all 16 EPA priority PAHs. Standard C18 columns may not resolve critical isomer pairs like benzo[b]fluoranthene/benzo[k]fluoranthene.
Which particle size should I choose?
5 μm (13021008) is the standard for conventional HPLC (≤4000 psi) and EPA compliance. 3 μm provides faster runs and higher resolution, suitable for UHPLC or modern HPLC systems (≥6000 psi). 10 μm is for preparative or high-loading applications.
Does this column replace Waters PAH C18 or Agilent ZORBAX Eclipse PAH?
Yes. It is compatible with the same mobile phase conditions (water/acetonitrile gradient) and dimensions (4.6×250 mm, 5 μm) as these columns.
Can this column be used with LC/MS?
Yes – use volatile mobile phase buffers (e.g., ammonium formate) instead of non-volatile phosphate buffers. 3 μm, 2.1 mm ID columns (13021001 or 13021002) are recommended for LC/MS to reduce flow rates and improve ionization efficiency.
Do you offer guard columns?
Yes – guard cartridges (4.6×10 mm, 5 μm) and holders are available for all 4.6 mm ID columns. Guard columns significantly extend analytical column lifetime, especially for complex matrices like soil extracts and wastewater samples.
Can you provide COA and test chromatogram before purchase?
Yes – Certificate of Analysis (COA) and a test chromatogram showing baseline separation of 16 EPA PAH standards are available upon request before purchase. Email info@hawach.com with the column part number.
Do I need to revalidate my EPA Method 610 when switching to this column?
For most environmental QC methods, no full revalidation is required. However, we recommend performing a system suitability test (e.g., 5-6 injections of an EPA 610 PAH standard mix) to confirm separation performance under your specific conditions. For regulated methods (e.g., EPA compliance testing, accredited labs), follow your internal change control protocol – typically minor verification with a reference standard is sufficient.
Is OEM / private label available?
Yes – private labeling, custom packaging, and bulk OEM manufacturing are available with minimum order quantity. Contact our OEM team at info@hawach.com with your specifications.
What detectors are compatible?
UV (254 nm) is sufficient for high-concentration PAH analysis. Fluorescence detection (FLD) provides significantly higher sensitivity for most PAHs (ng/L levels). LC/MS is suitable with volatile buffers and 2.1 mm ID columns.
Do you offer custom column dimensions?
Yes – custom dimensions (10-50 mm ID, non-standard lengths) and 300Å pore size are available with minimum order quantity. Contact OEM team.
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