Particle Size: 5 μm
Pore Size: 120Å
pH Stability: 1.5-10
Workflow: Pre-Column Derivatization
Method Package: Complete kit available
Availability: In Stock
Amino Acid HPLC Column
Description
Product Overview
Hawach Amino Acid HPLC column is a dedicated reversed-phase column specifically optimized for the separation of 18 common proteinogenic amino acids after pre-column derivatization. It is manufactured from ultra-high purity (>99.999 %) Type B silica with a unique bonding chemistry and double end-capping, ensuring excellent peak shape and reproducibility for polar amino acids.
Typical analysis time: 30 minutes per sample.
Supports multiple derivatization workflows:
- OPA– Fast, high sensitivity for primary amino acids
- PITC– Stable derivatives, covers all 18 amino acids
- FMOC / AQC– Optional protocols for extended coverage
Applications include: biopharmaceutical cell culture monitoring, protein hydrolysate analysis, food & feed nutritional profiling, and clinical diagnostics.
Key Features
- Dedicated amino acid column for 18-amino acid separation
- Ultra-high purity Type B silica (>99.999 %)
- Particle sizes: 5 μm
- Pore size: 120Å
- Wide pH stability: 1.5-10
- Double end-capping for long column lifetime
- Compatible with standard HPLC systems: Agilent, Waters, Shimadzu, Thermo
- Typical 30-minute run time for full 18-amino acid separation
- Complete method package available
Quick Decision Guide
| Application | Recommended Column | Particle / ID*Length | Method Package | Notes |
| Routine QC, 18 amino acids | 13021399 | 5 μm, 4.6*250 mm | Optional | Standard format, ready-to-use |
| Pre-column derivatization (OPA) | A13021399 | 5 μm, 4.6*250 mm | SLHAA-MP-OPA | Fast, high sensitivity for primary amino acids |
| Pre-column derivatization (PITC) | 13021399 | 5 μm, 4.6*250 mm | SLHAA-MP-PITC | Covers all 18 amino acids, stable derivatives |
| New method setup | 13021399 | 5 μm, 4.6*250 mm | Full kit | Column + Standards + Reagents + Protocol |
Compatibility with Industry Standard Methods
| Method | Column Used | Compatible? | Notes |
| Waters AccQ•Tag | Dedicated Amino Acid Column | Y | Equivalent performance, can substitute |
| Agilent Zorbax Eclipse AAA | Dedicated Amino Acid Column | Y | Direct replacement, comparable selectivity |
| Phenomenex Luna C18(2) | Dedicated Amino Acid Column | Y | Suitable alternative with same derivatization workflow |
| AOAC 2018.06 (animal feed proteins) | Dedicated Amino Acid Column | Y | Validated for protein hydrolysate amino acid determination |
| European Pharmacopoeia amino acid methods | Dedicated Amino Acid Column | Y | Compliant with pre-column derivatization approaches |
Supports derivatization methods:
- OPA -Primary amino acids
- FMOC -Secondary amino acids
- PITC -All 18 amino acids
- AQC -Full 18-amino acid kit
Chemical Compatibility & Mobile Phase Guidelines
Compatible with:
- Aqueous buffers (acetate, phosphate, formate, pH 1.5-5)
- Organic modifiers: acetonitrile, methanol, ethanol
- Derivatization reagents: OPA, FMOC, PITC, AQC, dansyl-Cl
- Typical mobile phase system: A -buffer/acetonitrile (93:7); B – acetonitrile/water (80:20)
Not recommended:
- Mobile phases without derivatization for UV detection
- 100% water mobile phase (use dedicated aqueous columns)
- Strong bases (>pH 10) -end-capping tolerates up to pH 10, alkaline conditions gradually degrade silica
Manufacturing & Quality System
- ISO 9001 certified
- Packed in Class 100,000 cleanroom
- High-purity bonding reagents, optimized for amino acids
- Each column batch QC-tested with 18-amino acid standard mix
- Full lot traceability with COA and test chromatogram
- OEM private label and custom dimensions available
Why choose Hawach Amino Acid HPLC columns
- Lower cost than major brands with equivalent or better separation of 18 amino acids
- Complete method package for labs without existing methods
- Direct replacement for common 18-amino acid pre-column derivatization workflows
- Pre-tested with standard mix -verified separation
- In-stock standard format: 4.6*250 mm, 5 μm (SKU: 13021399)
- Full documentation for GMP/GLP compliance
- OEM and distributor support -bulk pricing, private label, custom dimensions
Order Information
| Cat# | Description | Package (pcs/pk) |
| 13021399 | HPLC Column, Amino Acid, 5 μm, 120Å, 4.6*250 mm | 1 |
Typical Applications
| Industry | Sample Type | Purpose |
| Biopharma | Cell culture media, fermentation broth, protein hydrolysates | Monitor nutrient consumption, optimize growth, ensure product quality |
| Food & Beverage | Soy sauce, vinegar, fermented bean curds, nutritional supplements | Measure amino nitrogen, fermentation maturity, umami flavor |
| Feed | Animal feed, pet food, protein supplements | Verify protein quality, detect adulteration, ensure compliance |
| Pharmaceutical | Amino acid injections, drug formulations, QC testing | Ensure purity, potency, stability |
| Clinical Diagnostics | Plasma, serum, urine, dried blood spots | Screen metabolic disorders |
| Protein Research | Protein hydrolysates | Characterize protein identity & purity |
FAQ
3 μm vs 5 μm – which should I choose?
5 μm – conventional HPLC, longer lifetime, lower pressure. 3 μm – higher resolution, faster runs, requires higher pressure (≥ 6000 psi).
Can this column replace traditional ion-exchange amino acid analyzers?
Yes, for most QC and biopharma applications. It offers faster run times and lower instrument cost. For complex matrices or certain regulatory methods, ion-exchange may still be preferred.
Do I need a method package?
Recommended for first-time setups or method switches. Includes column, derivatization reagents, 18-amino acid standards, and protocol.
What is the lead time for Amino Acid HPLC columns?
Standard dimensions (4.6*250 mm, 5 μm) are in stock and ship within 3-5 business days. Custom dimensions or bulk orders require 2-3 weeks. Contact sales@hawach.com for current stock status.
Is OEM / private label available?
Yes. We offer private labeling, custom packaging, and bulk OEM manufacturing. Minimum order quantity applies. Contact our OEM team at sales@hawach.com with your specifications.
Can this column fully replace Waters AccQ•Tag column?
Yes. This dedicated Amino Acid column is a direct performance equivalent to Waters AccQ•Tag, Agilent Zorbax Eclipse AAA, and Phenomenex Luna C18 columns for 18-amino acid analysis. It uses the same AQC (or OPA/PITC) derivatization workflow and produces comparable resolution, retention times, and peak shapes.
Do I need to revalidate my method if I switch to Hawach column?
For most QC and routine methods, no full revalidation is required because the column meets USP L1 specifications and produces similar selectivity. However, we strongly recommend a simple system suitability test (e.g., 5-6 injections of an 18-amino acid standard) to confirm column performance under your specific conditions. For GMP methods, follow your internal change control protocol – typically minor verification is sufficient.
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