USP Class VI
Breathable or Sealed Cap
1 / 2 / 5 / 10 / 40 layers
656 – 25,295 cm² growth area
200 – 8,000 mL working volume
TC treated / Non-treated
Gamma sterilized
DNase/RNase free
Availability: In Stock
Multi-Layer Flask
Description
Designed for industrial-scale adherent and suspension cell culture in biopharmaceutical manufacturing, vaccine production, and monoclonal antibody development. Hawach multi-layer flasks (also known as multi-layered cell culture flasks) are available in 1, 2, 5, 10, and 40 layers. Manufactured from medical-grade USP Class VI polystyrene in a Class 10,000 cleanroom, gamma sterilized, and certified non-pyrogenic, DNase/RNase free. Ultrasonic welding and bonding technology ensures robust, leak-free construction while saving time and space.
Product Specifications – Multi-Layer Flasks
TC Treated – For Adherent Cell Culture
| Cat. No. | Layers | Growth Area (cm²) | Working Volume (mL) | Cap Type | Treatment | Packaging |
| 16051101 | 1 | 656 | 200 | 2 openings (sealed/breathable option) | TC treated | 4 pcs/carton |
| 16051102 | 2 | 1296 | 400 | 2 openings | TC treated | 4 pcs/carton |
| 16051103 | 5 | 3216 | 1000 | 2 openings | TC treated | 4 pcs/carton |
| 16051104 | 10 | 6416 | 2000 | 2 openings | TC treated | 2 pcs/carton |
| 16051105 | 10 | 6416 | 2000 | 2 openings | TC treated | 6 pcs/carton |
| 16051106 | 40 | 25295 | 8000 | 2 openings | TC treated | 2 pcs/carton |
Non-Treated – For Suspension Culture or Specialized Applications
| Cat. No. | Layers | Growth Area (cm²) | Working Volume (mL) | Cap Type | Treatment | Packaging |
| 16051201 | 1 | 656 | 200 | 2 openings | Non-treated | 4 pcs/carton |
| 16051202 | 2 | 1296 | 400 | 2 openings | Non-treated | 4 pcs/carton |
| 16051203 | 5 | 3216 | 1000 | 2 openings | Non-treated | 4 pcs/carton |
| 16051204 | 10 | 6416 | 2000 | 2 openings | Non-treated | 2 pcs/carton |
| 16051205 | 10 | 6416 | 2000 | 2 openings | Non-treated | 6 pcs/carton |
| 16051206 | 40 | 25295 | 8000 | 2 openings | Non-treated | 2 pcs/carton |
Download PDF Datasheet
Note: 10-layer flasks are offered in two pack sizes (2 or 6 units per carton) to accommodate different production scales. All flasks feature two openings for filling, sampling, and gas exchange.
How to Choose the Right Multi-Layer Flask – Selection Guide
Layer Count – Scale Your Culture
| Layers | Growth Area | Working Volume | Typical Application |
| 1 layer | 656 cm² | 200 mL | Lab-scale process development, small batches |
| 2 layers | 1296 cm² | 400 mL | Pilot runs, scale-up studies |
| 5 layers | 3216 cm² | 1000 mL | Medium-scale production |
| 10 layers | 6416 cm² | 2000 mL | Large-scale manufacturing, vaccine production |
| 40 layers | 25295 cm² | 8000 mL | Industrial mass production, monoclonal antibodies |
TC Treated vs Non-Treated
TC Treated (Tissue Culture treated): Surface modified by plasma or corona discharge to enhance hydrophilicity and cell attachment. Recommended for adherent cells (e.g., CHO, HEK293, MSCs, fibroblasts).
Non-Treated: Original hydrophobic surface. Recommended for suspension cells (e.g., hybridomas, lymphocytes, insect cells) or when cells do not require attachment.
Cap Options – Sealed vs Breathable
Sealed cap: Provides complete closure – suitable for closed system processing or when gas exchange is not critical.
Breathable cap (with hydrophobic membrane): Allows sterile gas exchange (CO₂/O₂) while preventing contamination and liquid spillage. Recommended for most cell culture applications requiring incubation with 5-10% CO₂. Please specify cap preference when ordering.
Applications – Large-Scale Cell Culture with Multi-Layer Flasks
Monoclonal Antibody Production
Ideal for CHO and hybridoma cell culture at 10-40 layer scale. High surface area maximizes antibody yield in a compact footprint.
Vaccine Manufacturing
Adherent Vero, MDCK, or MRC-5 cells for viral vaccine production. TC treated 5-40 layer flasks support high-density culture.
Cell & Gene Therapy
Scale-up of adherent stem cells (MSCs) or viral vector production in 1-10 layer flasks under GMP-like conditions.
Recombinant Protein & Biosimilar Development
Large-scale expression of recombinant proteins using HEK293 or CHO cells. 10-layer and 40-layer formats reduce handling steps.
Single-Layer Flask vs Multi-Layer Flask vs Cell Factory
Choose the Right Cell Culture Vessel for Your Scale
Why Choose Hawach Multi-Layer Flasks?
USP Class VI Medical Grade
High-molecular polystyrene – biocompatible, low leachables, suitable for GMP processes.
Class 10,000 Cleanroom
Manufactured in ISO 7 (Class 10,000) environment – ensures low particulate and consistent quality.
Ultrasonic Welding + Bonding
Robust, leak-free construction eliminates solvents or adhesives that could leach into culture.
Gamma Sterilized
Sterility assurance level (SAL) 10⁻⁶. Ready-to-use, non-pyrogenic, DNase/RNase free.
TC or Non-TC Options
One product line covers both adherent and suspension workflows.
Breathable Cap with Hydrophobic Membrane
Sterile gas exchange, prevents contamination – ideal for CO₂ incubators.
Technical Specifications – Multi-Layer Flask
| Parameter | Value |
| Material | Polystyrene (PS), USP Class VI medical grade |
| Layers available | 1, 2, 5, 10, 40 |
| Growth area per layer | approx. 632-656 cm² (depending on layer count) |
| Total growth area | 656 – 25,295 cm² |
| Working volume per layer | 200 mL (1-layer) to 200 mL per layer (10-layer = 2000 mL, 40-layer = 8000 mL) |
| Manufacturing environment | Class 10,000 (ISO 7) cleanroom |
| Sterilization | Gamma irradiation (SAL 10⁻⁶) |
| Pyrogen | Non-pyrogenic (endotoxin <0.5 EU/mL) |
| DNase / RNase | Free |
| Cap options | Sealed cap or breathable cap with hydrophobic membrane |
| Ports | 2 openings (filling and sampling/venting) |
Compatibility & Workflow Integration
Hawach multi-layer flasks are designed to fit standard cell culture incubators, biosafety cabinets, and automated handling systems. The compact footprint (same external dimensions for 1-10 layer units) allows easy scale-up without changing equipment. Breathable caps ensure compatibility with humidified CO₂ incubators (5-10% CO₂, 37°C). For GMP manufacturing, we provide batch-specific certificates of analysis, irradiation certificates, and sterility test reports.
Frequently Asked Questions
What is the difference between TC treated and non-treated multi-layer flasks?
TC treated flasks have a hydrophilic surface modified for optimal cell attachment – essential for adherent cells (e.g., CHO, Vero, MSCs). Non-treated flasks retain the original hydrophobic surface and are suitable for suspension cells (e.g., hybridomas, lymphocytes) or applications where attachment is not required.
What is the minimum order quantity (MOQ)?
MOQ is 1 carton (pack sizes vary: 2, 4, or 6 units per carton). Sample units (1-2 flasks) are available for evaluation.
Can I get free samples?
Yes – we offer limited sample units for process validation. Shipping fee may apply.
Are these flasks compatible with robotic liquid handlers?
Yes – the standard footprint and dual-opening design allow automated filling, sampling, and harvesting. We recommend compatibility testing with your specific system.
What cap options are available?
Both sealed caps and breathable caps (with hydrophobic membrane for sterile gas exchange) are available. Please specify your preference when ordering.
Can I use these flasks for suspension cells?
Yes – choose non-treated version for suspension cells. For high-density suspension, consider our breathable cap option to maintain proper gas exchange.
How do I know which layer count to choose?
Start with 1-layer for process development, then scale vertically using the same footprint. 5-10 layers for pilot batches, 40 layers for full-scale production. Contact our application specialists for scale-up guidance.
Can I get OEM labeling or custom packaging?
Yes – private label, custom barcoding, and non-standard pack sizes are available for volume orders. MOQ for OEM: 50 units per SKU.
Trust & Support – Why Hawach
- Class 10,000 (ISO 7) cleanroom -no cross-contamination risk
- USP Class VI polystyrene -biocompatible for pharmaceutical use
- Gamma irradiation validated -SAL 10⁻⁶
- Global supply chain -stock in US, Europe, Asia
- Technical support for scale-up, process optimization, and regulatory documentation
The quality has a guarantee
Fast & safe shipping worldwide
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