Column: XChroma HPLC Column, C18-Universal, 5μm, 4.6×250mm
Mobile Phase: (D-Phenylalanine(PHE) 1.32g, CuSO4 1.00g to 1000mL water, adjust pH=3.50 with NaOH)/methanol=82/18
Wavelength: 293nm
Temp.: 35°C
Flow Rate: 1.0mL/min