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The basic principle of QuEChERS method and its application

In recent years, the QuEChERS method has been widely used in the pretreatment of food safety test samples. Food safety testing is also a major issue related to the international people’s livelihood. Therefore, this paper describes the basic principles of the QuEChERS method and its application in the pretreatment of food safety test samples. Made a discussion.

QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) is a recent rapid development of a rapid sample preparation technology for agricultural product testing in the world. It was developed by Professor Anastassiades of the US Department of Agriculture in 2003. Pre-treatment is the longest and most labor-intensive part of food testing. The quality of pre-treatment determines the accuracy and precision of the analysis. The QuEChERS method has become a well-received new technology for sample preparation due to its Quick, Easy, Cheap, Effective, Rugged, Safe features.

1. The basic principle of the QuEChERS method
The principle is similar to high performance liquid chromatography (HPLC) and solid phase extraction (SPE). It uses the adsorbent filler to interact with impurities in the matrix to adsorb impurities to achieve impurity removal. Specifically, after the homogenized sample is extracted by acetonitrile (or acidified acetonitrile), the salt is separated and layered by extraction salt, and the matrix dispersive extraction mechanism is used, and PSA or other adsorbent and most of the interfering substances in the matrix are used (organic Acid, fatty acid, carbohydrate, etc. are combined and removed by centrifugation to achieve purification.
2. Application in pretreatment of food safety testing samples
There are many applications in the QuEChERS method. As long as the recovery rate of the target to be tested meets the demand, and the matrix background of the impurity removal meets the detection requirements, the method can be used to purify the pesticide residues, animal residues, additives, and illegal addition of food. All areas of materials and plasticizers may be involved. However, the current QuEChERS method is mainly used in the detection of pesticide residues in agricultural products.

2018-09-07T02:47:55+00:00September 7th, 2018|