The Application and Characteristics of Liquid Chromatography Column
The application and characteristics of the liquid chromatography column is a liquid chromatography instrument. Before choosing a column, first understand your own samples and impurities, their type structure, polarity, acidity and alkalinity, molecular weight, etc.
1. The sample is polar and weakly acidic; you can choose C18 at 100 Under the condition of acidic aqueous solution, it is necessary to select a column that withstands 100% pure water and retains a good polarity for the polar compound.
2. If the sample is too polar or too acidic; you can choose CN, NH2, or silica gel column. HILIC (Hydrophilic Chromatography), also use strong anion exchange chromatography column (the disadvantage is that the ion pair reagent has a long equilibrium time and the mobile phase pH requirement is relatively precise, otherwise it is difficult to repeat the experiment, and the ion pair reagent is difficult. Washed down, basically the column with ion pair can not be used for other experiments)
3. If the basic compound is too polar or too alkaline; you can choose a wide pH C18 column at high pH detection (the advantage is that the method development is simple, the disadvantage is that the current column of the technology to achieve this technology is relatively small, the price is also high) or the use of HILIC column (silicone column used in reversed phase conditions, which is also very The method of detecting alkaline samples). Select a strong ion exchange column (the disadvantage is that it can not be used to analyze other samples, the pH of the mobile phase is relatively precise, otherwise it is difficult to repeat the experiment).