Sudan Red Liquid Chromatography Detection Method and Experiment
Sudan red liquid chromatography detection method:
The national standard for Sudan red liquid chromatography uses a simple principle of positive phase adsorption solid phase extraction to remove the interference components in the red pepper and tomato in the sample at one time. It can be accurately determined by the high-performance liquid chromatograph that has been widely used in China. The detection of four Sudan red dyes was completed. The scientific method flow was determined when the standard was developed. This process is also the test procedure that should be followed in the actual test, ie sample preparation – sample – dispersion – extraction – centrifugation – separation supernatant – evaporation dry – n-hexane dissolution – solid phase Extraction – elution – constant volume – on the machine. According to reports, this kind of detection is easy to operate. As long as the corresponding instruments are equipped, the key is high-performance liquid chromatography. The testers of technical structures above the county level in China can effectively detect the Sudan red content in the food according to the standard.
Sudan red liquid chromatography test
1, the scope of application
Sudan red liquid chromatography detected the detection of the main components of peppers, including Sudan Red No. 1, Sudan Red No. 2, Sudan Red No. 3, Sudan Red No. 4, Sudan Orange B, Sudan Red 7B and Carmine Orange.
2, the definition
Sudan Red is a non-biosynthetic colorant used in chemical products such as oil paint, wax, floor wax, and soap. It is generally insoluble in water and soluble in organic solvents. Carmine orange is a food coloring agent but is not allowed in Used in paprika and condiments.
3, method points
The above coloring agent was extracted with acetonitrile, filtered, and the filtrate was subjected to chromatography by reversed-phase high-performance liquid chromatography. Qualitative and quantitative with a wavelength-variable UV-visible detector.
4, reagents and standards
Unless otherwise specified, the reagents involved in the method are of analytical grade, and the experimental water is distilled water, deionized water or analytical water of the same quality.