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Polar-RP HPLC Columns

Column: XChromaTM HPLC Column, Polar-RP, 5μm, 4.6×250mm
Mobile Phase: Buffer Solution/methanol/water=10/5/85
Wavelength: 254nm
Temperature: 35°C
Flow Rte: 1.5ml/min
Injection Volume: 20μL



Description of Polar-RP HPLC columns

The RP HPLC Column of Hawach has a polar functional group, which is close to the phase of improving wettability, thereby reducing the disintegration. The bonding process is used to solve the problem of residual amino groups, and asymmetrical peak shape is obtained by using double end cap technology. It also protects the unreactive silanol group, providing extreme peaks and different selectivity for polar and strongly alkaline compounds, rather than the C18 phase.


Double end-capping technology, good chemical stability guaranteed.
Sample size and pore size are uniforms to ensure well flow rate.
Peak shape and peak volume are under the normal standard.
High chemical inertness, durability, and reproducibility ensured.
Can separate fast with a reasonable separation pressure.
The influence of raw material silicone is minimized.
Strong interaction with silica gel surface while separating leads to an excellent separation effect of the compound.
Wide range of pH with long service life.
Applied with most HPLC instruments.

Features of Polar-RP HPLC columns

Using ultra-pure silica gel as the matrix
Double end-capping technology to ensure excellent symmetry peak
pH Range: 1.5-10.0 Use 100% water for flow phase
The properties of strong polarity and alkaline structure have good retention and peak symmetry

Polar-RP HPLC Columns

Applications of Polar-RP HPLC columns

Determination of the contents of puerarin, daidzin, and daidzein in roots stems and leaves of Pueraria lobata.


Ordering Information

Polar-RP HPLC Columns_OI
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