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NH2 HPLC Columns

Column: XChroma HPLC Column, NH2, 5μm, 4.6×150mm
Wavelength: 210nm
Mobile Phase: Acetonitrile/phosphate buffer = 70/30
Temperature: 35°C
Flow Rate: 1.8mL/min
Injection Volume: 20μL



Description of NH2 HPLC Columns

Hawach Scientific Co., Ltd NH2 stationary phase of HPLC Column is based on ultra pure porous spherical silica gel matrix, by using the stationary phase bonding technology, using the contain aminopropyl functional groups of organic silane bonded, can faster than silica gel column balance, is sensitive to the water content of mobile phase without silica gel column. Most of the time, NH2 columns are used in normal phase LC analysis.



End-capping technology ensures high chemical stability.
More uniform aperture because of the unique silica gel matrix treatment technology.
Reasonable sample size & pore size make the good flow rate.
Peak volume is high and peak shapes perfect.
Ultra inertness, compatible with a wide range of HPLC instruments.
Separation rate is reasonable.
The influence of raw material silicone is greatly reduced.
pH ranges widely with a long service life

Features of NH2 HPLC Columns

For applications in aggressive normal phase mode with an aqueous eluent
Can separate Vitamins A and D in the normal-phase mode
Carbohydrates and monosaccharide can be separated in the reversed-phase mode on NH2, but not suited for the separation of compounds and sugars that contain aldehyde, carbonyl
Able to separate some negatively-charged molecules for weak anion exchange mechanism in the low pH buffer solution.

Applications of NH2 HPLC Columns

1. It is widely used in the analysis of low molecular weight sugars such as glucose, fructose, xylose, and lactose.
2. Suitable for most of the positive phase chromatography applications
3. Normal phase analysis, weak anion exchange and reversed phase analysis of water containing polar compounds
4. Positive, reverse, weak anion exchange


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