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C18 Low pH HPLC Columns

Column: XChroma HPLC Column, C18-Low pH, 5μm, 4.6×250mm
Wavelength: 252nm
Mobile Phase: Methanol/water=5/95
Temp.: Ambient 25°C
Flow Rate: 1.0mL/min
Injection Volume: 20μL



Description of C18 Low pH HPLC Columns

Hawach Scientific Co., Ltd C18-Low pH compatible C18 phases are designed for use at extremely low pH. Two very bulky hydrophobic protective groups prevent siloxane bond from hydrolysis at low pH condition and at high temperature, making it the most stable C18 for low pH application in the market. Because KL phase is not end-capped and has more surface silanols, it retains early-eluting polar compounds better.


C18 Low pH can apply for 100% water as the mobile phase
Ultra-pure all-porous silica gel, pure > 99.999%
pH Range: 0.5-8.0
Highest use temperature is 70 °C
Good peak symmetry: the drag tail factor is controlled at 0.95-1.05
Particle size: 120 A, 300A for selection
300A C18-Low pH can separate large molecular organisms. Such as polypeptides and proteins.

Advantages of C18 Low pH HPLC Columns

Well flow rate due to its average sample size and pore size.
Pure silica gel ensures high mechanical strength and high specific surface area.
Spherical silica gel, good stability, improve separation efficiency and reproducibility
Ultra-dense bonding and double-ended ensure long life
Basic, acidic, and neutral compounds make excellent peak shape
pH range from 0.5 to 8.0, wide choice.
Used with most HPLC instruments


It is suitable for the analysis of polypeptides and proteins.


Ordering Information

C18 Low pH HPLC Columns
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