Sterilization and Disinfection of Pipette Tips

How to sterilize pipette tips? The tip sterilization of pipette tips is as follows.

Put the pipette tip in the tip box and sterilize it with moist heat to avoid water vapor troubles. Newspapers can be wrapped around the tip box or placed in an incubator to dry after sterilization. The tip box can be wrapped in newspaper for autoclaving, and newspaper bags can be used to avoid too much water, absorb water, and the most importantly thing is to prevent re-contamination. But if it is not packed, it will not have much effect. After the sterilization is completed, if there is more water, you can put it in the oven for a longer time.

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1. Matters needing attention in sterilizing pipette tips during RNA extraction?

How to autoclave pipette tips?

  • Ordinary Sterilization
    • Before high-pressure sterilization, soak the ordinary EP tube and gun tip in DEPC water overnight to remove RNase. After removing DEPC the next day, put them in the pipette tip box for moist heat sterilization. Domestic tubes should still be sterilized; otherwise, they are prone to degradation, especially some cells or tissues with high RNase content.
    • Although some people did not use DEPC water to treat the pipette tip, and the RNA extraction result page is not bad, this is not true. It is recommended that you still operate steadily to avoid the experiment, and do not know where to find the cause, except for the problem.
  • High-Pressure Sterilization
    • The pipette tip is autoclaved and sterilized. The high-temperature and high-pressure sterilization of pipettes is divided into whole sterilization and half sterilization. Most brands of pipettes can be half-sterilized, but whether they can be sterilized as a whole, users must contact the supplier quotient confirmation. In the market, products such as manual pipettes can be sterilized as a whole. But here are a few points to remember:
    • First, except for individual applications, such as infectious virus research, most customers do not need to sterilize the entire tube. Second, for products of the same brand, it is often more expensive to sterilize the entire tube than half. For bacteria, customers need to decide according to their own needs.

10ul pipette tips 200ul yellow pipette tips 1250ul filtered pipette tips

2. Should the entire pipette be sterilized when extracting RNA?

The pipette generally does not need to be sterilized. If it is needed, how do you autoclave pipette tips? Only put it on the ultra-clean workbench and irradiate it with ultraviolet light for half an hour.

3. What precautions should be taken when sterilizing pipette tips?

  • a. Choose the appropriate sterilization method: Choose the proper sterilization method according to the material of the pipette tip, experimental requirements, and laboratory conditions.
  • b. Ensure the sterilization effect: Operate according to the requirements of the sterilization method to ensure that parameters such as sterilization time and temperature meet the standards.
  • c. Prevent re-contamination: To avoid re-contamination, the sterilized pipette tip should be stored and used under sterile conditions.
  • d. Safe operation: When performing high-temperature and chemical sterilization, ensure safe operation to avoid burns and chemical contact with the skin or eyes.

4. Advantages of high-temperature steam sterilization

Steam heat penetration is strong; sterilization efficiency is high; sterilization time is short; no chemical or physical pollution during sterilization; sterilization equipment has few control parameters and stable operation; steam sterilization is used for water and energy saving. High thermal efficiency.