Principles for Selecting SPE Cartridges Specifications

The principle of selecting the SPE cartridges specification is to first ensure that the SPE cartridges packing has sufficient capacity to retain the target compound 100% on the column. On the other hand, the filler is not too much. The more filler, the greater the amount of solvent that is effective for the target compound to elute. You also can choose Reversed Phase C18 SPE Cartridges, SCX SPE Cartridges, SAX SPE Cartridges, Normal Phase PR Grade Florisil SPE Cartridges and other SPE Cartridges using.

Therefore, when selecting the specifications of the SPE cartridges, the SPE cartridge’s capacity must be considered. Cartridge capacity refers to the mass of a compound that retains a compound with a primary force per unit mass. The capacity of the bonded silica cartridges is generally from 1% to 5%. Take the SPE cartridges with 100 mg filler as an example, its larger capacity generally does not exceed 5 mg. The cartridge capacity of the ion exchanger is about 0.5 to 1.5 meq/g.

It is worth pointing out that because we are mostly a mixture, there are many interfering substances besides the target compound. Therefore, when selecting the size of the cartridges, it is necessary to consider the target compound and the total amount of interference substances that may be adsorbed under the extraction conditions, and not only the target compound.

Otherwise, even if the target compound does not exceed the capacity of the cartridge, the SPE cartridges retain some of the interfering substances while retaining the target compound. Since the total amount of the two has exceeded the capacity of the cartridge, the result is that some of the target compounds cannot be retained. On the SPE cartridges. Therefore, for the sake of safety, it is recommended that the capacity of the cartridge be better than twice the estimated target compound content.

Construction-of-SPE-cartridge

The Structure of SPE Cartridge

1. Tube
The tube is the carrier of the adsorbent. Basically, it is made of serum polypropylene, and always in the shape of the injection syringe. Some tubes are also made of glass and used for special analysis. The sizes of the bottom outlet of the tube have been standardized, so they can be applied to different SPE multitube vacuum devices.

2. Sieve Plate
The sieve plate is used for fixing the adsorbent and filtering the solvent. Polyethylene is a common material for making sieve plates. As for special analysis, materials such as Poly Tetra Fluoro Ethylene (PTFE), stainless steel sheets, and glass.

3. Adsorbent
The adsorbent is used for separation in the SPE cartridge. The adsorbents including aluminum oxide, florist, and graphitized carbon black which are widely applied in column chromatography at an early stage, can still be used in solid-phase extraction.

Currently, the most common adsorbent is silica gel adsorbent. However, the organic polymer adsorbent with the advantages of good reproducibility, wide pH range of application, and wide applicability, has been a substitute for the silica gel adsorbent in many applications.

4. Assorted Device
It is used for increasing the volume of the container above the tube and then improving the single loading quantity of the sample.

Three Advantages of SPE Cartridge

Hawach’s SPE series of solid-phase extraction products are produced according to strict quality control standards. Determination of surface characteristics such as pore size, pore volume, specific surface area, and carbon loading of raw materials and semi-finished fillers. Iwate, the substitution of clean production textile contaminant levels, flow rate measurement under constant elution conditions, and accurate weighing of packed packing.

A series of high-standard quality assurance measures ensure the quality of Hawach’s SPE series of solid-phase extraction products. The performance of separation and extraction is good, and the recovery rate is relatively high and constant.

The SPE column is based on unbonded active irregular silica gel (silica gel-based) particles. The main interaction is a polar interaction (such as a hydrogen bond). Since the silanol group can be ionized, it can be used as a weak cation exchanger in the medium pH range.

  • Anti-cross-contamination, anti-atomization vacuum tank design
  • Can be equipped with a large-capacity sampler, fast concentrating and drying device, batch processing sample
  • SPE cartridge is stable in quality, high in the sample recovery rate, and good in precision