Cellulose Acetate Membrane Electrophoresis

Cellulose acetate membrane electrophoresis is supported by cellulose acetate membrane with the advantages of easy operation, fast and cheap. It is soluble in acetone and other organic solutions, which can be coated into a fine microporous membrane, whose thickness of 0.1mm – 0.15mm is appropriate.

Application

It has been widely used in the separation and analysis of serum protein, hemoglobin, globulin, lipoprotein, sugar egg white, alpha-fetoprotein, a steroid hormone, and isozyme. Although its resolution is lower than that of polyacrylamide gel electrophoresis, it has the advantages of simplicity and rapidity.

Feature

1. The accuracy of analyzing and separating protein samples is high.
2. Fast separation speed, and short electrophoresis time. The whole electrophoresis is only about 90 minutes.
3. It has wide applications.
4. It is good for scanning quantification and long-term preservation.

The disadvantages of membrane electrophoresis are small thickness, small sample dosage, and not suitable for preparation.

Power selection

The appropriate current should be selected in the cellulose acetate membrane electrophoresis process. High currents can cause protein denaturation. Low current can cause the swimming speed of the sample is slow and easy to diffuse.