How To Choose The Extract Solvents And Purifiers In QuEChERS Method
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Originally designed for the analysis of fruits and vegetables, QuEChERS is well known as a “Quick, Easy, Cheap, Effective, Rugged and Safe”sample extraction and clean-up technique. It is widely used for the analysis of multiple residues, due to the emerging need to determine trace amounts of analytes in agricultural industry.
Generally, QuEChERS technique has two stages to complete the analysis. In the first stage, through of process of adding an organic solvent and a blend of salts which allow the normally miscible organic solvent to separate from the water, you can find sample extraction Analytes of interest being extracted from the sample .
After that, we use centrifugation to separate the organic phase from the aqueous phase and the sample solids, allowing for easy subsampling of the extract. The second stage is to cleanup the subsample of the organic solvent extract from the first Stage, by using a dispersive solid phase extraction media (D-SPE).
HAWACH QuEChERS extraction and purification kit
HAWACH provide extraction kits with or without 50ml centrifuge tubes and lids, including MgSO4, NaCl or other buffer salts. HAWACH pre-packages the required QuEChERS salts and buffers in anhydrous packaging, so that you can add these salts and buffers after adding the solvent to the sample as required by the QuEChERS method.
Purification kit is the adsorbent is directly installed in 2ml, 15ml centrifuge tubes, and its role is to use the purification of the extracted mixture to remove impurities.
Selection of extraction solvents and purifiers
The choice of extraction solvent and purification agent is a key part of the QuEChERS method. Compared with solvents such as acetone and ethyl acetate, acetonitrile is used as the extraction solvent, and the interference components of the extracted product are less, and it can be easily separated from the aqueous phase through salting out.
Therefore, acetonitrile is a commonly used extraction solvent in the QuEChERS method. The acetonitrile mixed with water or adding a certain amount of acid is also commonly used extraction solvent, but as the solvent volume increases, the recovery rate of the analyte will tend to increase to a certain level, so the extraction solvent volume usually needs to be optimized.
Acetonitrile containing acetic acid can increase the recovery of ractopamine in pork and sulfonamides in chicken, while the use of formic acid in acetonitrile can increase the recovery of quinolone in fish. In the sample purification process, the choice of adsorbent must be based on the characteristics of the analyte and the sample matrix.
New adsorbents such as Z-Sep and Z-Sep + are mixed with C18 and PSA, which can effectively improve the purification efficiency of high-fat samples such as meat, eggs and milk. Nanotechnology has also been applied in the QuEChERS method, and there have been studies using ZnO nanoparticles and PSA as adsorbents for purifying milk and honey samples in the analysis of steroid drug residues, and achieved good recovery rates.
To meet the demands of your particular sample, Hawach porvides high quality QuEChERS extraction salts, QuEChERS Ceramic Homogenizers, QuEChERS dispersive absorbent, QuEChERS D-SPE Kits and QuEChERS Kits, always helping you get the better results.